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Chemical Genomics: Reviews and Protocols

Reviews and Protocols. 'Methods in Molecular Biology'. Auflage 2005. Sprache: Englisch.
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Chemical genomics is an exciting new field that aims to transform biolo- cal chemistry into a high-throughput industrialized process, much in the same way that molecular biology has been transformed by genomics. The inter- tion of small organic molec … weiterlesen
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Titel: Chemical Genomics: Reviews and Protocols

ISBN: 1588293998
EAN: 9781588293992
Reviews and Protocols.
'Methods in Molecular Biology'.
Auflage 2005.
Sprache: Englisch.
Herausgegeben von Edward D. Zanders
SPRINGER VERLAG GMBH

1. August 2005 - gebunden - 280 Seiten

Beschreibung

Chemical genomics is an exciting new field that aims to transform biolo- cal chemistry into a high-throughput industrialized process, much in the same way that molecular biology has been transformed by genomics. The inter- tion of small organic molecules with biological systems (mostly proteins) underpins drug discovery in the pharmaceutical and biotechnology industries, and therefore a volume of laboratory protocols that covers the key aspects of chemical genomics would be of use to biologists and chemists in these orga- zations. Academic scientists have been exploring the functions of proteins using small molecules as probes for many years and therefore would also b- efit from sharing ideas and laboratory procedures. Whatever the organizational backgrounds of the scientists involved, the challenges of extracting the ma- mum human benefit from genome sequencing projects remains considerable, and one where it is increasingly recognized that chemical genomics will play an important part. Chemical Genomics: Reviews and Protocols is divided into two sections, the first being a series of reviews to describe what chemical genomics is about and to set the scene for the protocol chapters. The subject is introduced by Paul Caron, who explains the various flavors of chemical genomics. This is f- lowed by Lutz Weber and Philip Dean who cover the interaction between organic molecules and protein targets from the different perspectives of la- ratory experimentation and in silico design. The protocols begin with the me- ods developed in Christopher Lowes' laboratory (Roque et al.

Inhaltsverzeichnis

Part I. Reviews

Introduction to Chemical Genomics
Paul R. Caron

Chemistry for Chemical Genomics
Lutz Weber

Computer-Aided Design of Small Molecules for Chemical Genomics
Philip M. Dean

Part II. Protocols

Design, Synthesis, and Screening of Biomimetic Ligands for Affinity Chromatography
Ana Cecília A. Roque, Geeta Gupta, and Christopher R. Lowe

The Role and Application of In Silico Docking in Chemical Genomics Research
Aldo Jongejan, Chris de Graaf, Nico P. E. Vermeulen, Rob Leurs, and Iwan J. P. de Esch

Synthesis of Complex Carbohydrates and Glyconjugates: Enzymatic Synthesis of Globotetraose Using b-1,3-N-Acetylgalactosaminyltransferase LgtD From Haemophilus infuenzae Strain Rd
Kang Ryu, Steven Lin, Jun Shao, Jing Song, Min Chen, Wei Wang, Hanfen Li, Wen Yi, and Peng George Wang

High-Throughput Cloning for Proteomics Research
Sharon A. Doyle

Screening for the Expression of Soluble Recombinant Protein in Escherichia coli
Sharon A. Doyle

High-Throughput Purification of Hexahistidine-Tagged Proteins Expressed in E. coli
Michael B. Murphy and Sharon A. Doyle

The Wheat Germ Cell-Free Expression System: Methods for High-Throughput Materialization of Genetic Information
Tatsuya Sawasaki, Mudeppa D. Gouda, Takayasu Kawasaki, Takafumi Tsuboi, Yuzuru Tozawa, Kazuyuki Takai, and Yaeta Endo

Advances in Genome-Wide Protein Expression Using the Wheat Germ Cell-Free System
Yaeta Endo and Tatsuya Sawasaki

Production of Proteins for NMR Studies Using the Wheat Germ Cell-Free System
Toshiyuki Kohno

Adenoviral Expression of Reporter Proteins for High-Throughput Cell-Based Screening
Jon Hoyt and Randall W. King

Fabrication of Protein Function Microarrays for Systems-Oriented Proteomic Analysis
Jonathan M. Blackburn and Darren J. Hart

Peptideand Small-Molecule Microarrays
Jan Marik and Kit S. Lam

Peptide Mass Fingerprinting: Protein Identification Using MALDI-TOF Mass Spectrometry
Judith Webster and David Oxley

A Practical Protocol for Carbohydrate Microarrays
Ruobing Wang, Shaoyi Liu, Dhaval Shah, and Denong Wang

Development of a Yeast Two-Hybrid Screen for Selection of Human Ras-Raf Protein Interaction Inhibitors
Vladimir Khazak, Erica A. Golemis, and Lutz Weber

Index

Pressestimmen

"...an elaborate combination of scientific background information with well-illustrated protocols...a prime reference book for scientists." -ChemBioChem

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