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Colorectal Cancer

Methods and Protocols. 'Methods in Molecular Medicine'. Auflage 2001. Book. Sprache: Englisch.
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The contents of Colorectal Cancer: Methods and Protocols aim to instruct investigators in all the key genetic, cellular, and molecular biological methods of analyzing colorectal tumors. The focused techniques and assays are described in sufficient de … weiterlesen
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Produktdetails

Titel: Colorectal Cancer

ISBN: 0896037673
EAN: 9780896037670
Methods and Protocols.
'Methods in Molecular Medicine'.
Auflage 2001.
Book.
Sprache: Englisch.
Herausgegeben von Steven M. Powell
Humana Press

13. Oktober 2000 - gebunden - 312 Seiten

Beschreibung

The contents of Colorectal Cancer: Methods and Protocols aim to instruct investigators in all the key genetic, cellular, and molecular biological methods of analyzing colorectal tumors. The focused techniques and assays are described in sufficient detail to allow researchers to start an experiment on colon tumors and proceed from beginning to end as if the expert in the field who has performed these studies were guiding them at the bench. Of note, most of the chapters in this volume are written by those scientists who p- neered these methods and assays in their respective fields. The chapters in Colorectal Cancer: Methods and Protocols describe "state of the art" methods to analyze colorectal tumors, ranging from gross mic- dissection of specimens to specific molecular analyses. Included are coverages of mutational assays, instability testing, immunohistochemical assays, chro- somal studies, and gene expression analyses. The goal of our volume is to facilitate the performance of colorectal tumor biological experiments by investigators at various levels of training-from graduate students and postdoctoral fellows to principal investigators who desire to advance our understanding of colon cancer development.

Inhaltsverzeichnis

Microdissection of Histological Sections: Manual and Laser Capture Microdissection Techniques
Christopher A. Moskaluk

Isolation of a Purified Epithelial Cell Population from Human Colon
James K. Roche

Xenografting Human Colon Cancers
Jeffrey C. Harper, Reid B. Adams, and Steven M. Powell

Comparative Genomic Hybridization Technique
Wa'el El-Rifai and Sakari Knuutila

Fluorescence In Situ Hybridization: Application in Cancer Research and Clinical Diagnostics
Svetlana D. Pack and Zhengping Zhuang

Microsatellite Analysis to Assess Chromosome 18q Status in Colorectal Cancer
Jin Jen

The SURF Technique: Selective Genetic Analysis of Microscopic Tissue Heterogeneity
Darryl Shibata

Microsatellite Instability Testing
Yann R. Parc and Kevin C. Halling

Immunohistochemical Analysis for hMLH1 and hMSH2 Expression in Colorectal Cancer
Kevin C. Halling and Patrick C. Roche

Mutation Detection in Colorectal Cancers: Direct Sequencing of DNA Mismatch Repair Genes
Julie M. Cunningham, David J. Tester, and Stephen N. Thibodeau

Mutation Detection in the TGF-b Receptors
William M. Grady, Lois L. Myeroff, Hongmei He, and Sanford Markowitz

Direct Analysis for Familial Adenomatous Polyposis Mutations
Steven M. Powell

I1307K Mutation Detection by Allele-Specific PCR in Familial Colorectal Cancer
Perry S. Chan and Steven L. Gersen

Microsatellite Analysis of the Insulin-Like Growth Factor II Receptor in Colorectal Carcinomas
Rhonda F. Souza and Stephen J. Meltzer

Molecular Detection of Smad2/Smad4 Alterations in Colorectal Tumors
Sam Thiagalingam

Direct Sequencing for Juvenile Polyposis Gene SMAD4/DPC4 Mutations
Lauri A. Aaltonen and Stina Roth

Direct Sequencing for Peutz-Jehgers Gene LKB1 (STK11) Mutations
Lauri A. Aaltonen and Egle Avizienyte

Direct Sequencing for Cowden Syndrome Gene PTEN (MMAC1) Mutations
Lauri A. Aaltonen, Stina Roth, and Charis Eng

Genetic Analysis Using Microarrays
Wa'el El-Rifai and Sakari Knuutila

Cyclooxygenase-2 (COX-2) Protein Expression by Western Blotting
Hossam M. Kandil and Raymond N. DuBois

The Immunohistochemical Method
Lisa A. Cerilli and Henry F. Frierson, Jr

Immunohistochemical Detection and Quantitation of Cell Surface Receptors for Prostanoids
Rosana Cosme and James K. Roche

Adhesion Signaling Through Integrins
Reid B. Adams and Joshua D. Rovin

Electrophoretic Mobility Shift Assay (EMSA)
Michael F. Smith, Jr. and Sandrine Delbary-Gossart

Transfection Assays for Transformation of Model Colonic Cell Lines
Mark T. Worthington and Roger Qi Luo

Assessment of Intestinal Stem Cell Survival Using the Microcolony Formation Assay
Kimberly S. Tustison, Joanne Yu, and Steven M. Cohn

Gene Transfer into the Colonic Mucosa
Baçak Çoruh and Theresa T. Pizarro

Index

Pressestimmen

"This is quite interesting and a valuable volume. It aims to instruct investigators in the key genetic, cellular and molecular biological methods for analyzing colorectal tumors. It's quite precise in its description of these techniques and, as the preface says, "The focused techniques and assays are described in sufficient detail to allow researchers to start an experiment on colon tumors and proceed from beginning to end as if the expert in the field who has performed these studies were guiding them at the bench." A large amount of practical information is packed into one small volume and this volume is ideal for students and fellows who wish to expand their knowledge in this area and have a ready volume reference in developing new techniques...The volume is also good value for those of us who don't get into the lab as it clarifies many of the techniques which are used and helps the non-involved reader to understand the terminology and techniques used in this important area of cancer research." - Cancer Forum "...Dr. Saltz has produced as concise a presentation as possible within the framework of the widely scattered disciplines searching the complexity of diagnostic and therapeutic problems occurring among patients with colorectal cancer." - Comprehensive Therapy
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