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Developmental Biology Protocols als Buch

Developmental Biology Protocols

Volume I. 'Methods in Molecular Biology'. Auflage 2000. Book. Sprache: Englisch.
Buch (gebunden)
Developmental biology is one of the most exciting and fast-growing fields - day. In part, this is so because the subject matter deals with the innately fascinating biological events-changes in form, structure, and function of the organism. The other ... weiterlesen


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Developmental Biology Protocols als Buch
Titel: Developmental Biology Protocols

ISBN: 0896038521
EAN: 9780896038523
Volume I.
'Methods in Molecular Biology'.
Auflage 2000.
Sprache: Englisch.
Herausgegeben von Cecilia W. Lo, Rocky S. Tuan
Humana Press

19. November 1999 - gebunden - 612 Seiten


Developmental biology is one of the most exciting and fast-growing fields - day. In part, this is so because the subject matter deals with the innately fascinating biological events-changes in form, structure, and function of the organism. The other reason for much of the excitement in developmental biology is that the field has truly become the unifying melting pot of biology, and provides a framework that integrates anatomy, physiology, genetics, biochemistry, and cellular and molecular biology, as well as evolutionary biology. No longer is the study of embryonic development merely "embryology." In fact, development biology has produced important paradigms for both basic and clinical biomedical sciences alike. Though modern developmental biology has its roots in "experimental embry- ogy" and the even more classical "chemical embryology," the recent explosive and remarkable advances in developmental biology are critically linked to the advent of the "cellular and molecular biology revolution." The impressive arsenal of expe- mental and analytical tools derived from cell and molecular biology, which promise to continue to expand, together with the exponentially developing sophistication in fu- tional imaging and information technologies, guarantee that the study of the devel- ing embryo will contribute one of the most captivating areas of biological research in the next millennium.


Part I. Introduction. Developmental Biology Protocols: Overview I, Rocky S. Tuan and Cecilia W. Lo.
Part II. Systems: Production, Culture, and Storage. Rearing Larvae of Sea Urchins and Sea Stars for Developmental Studies, Christopher J. Lowe and Gregory
A. Wray. Large-Scale Culture and Preparation of Sea Urchin Embryos for Isolation of Transcriptional Regulatory Proteins, James A. Coffman and Patrick S. Leahy. The Chick Embryo as a Model System for Analyzing Mechanisms of Development, Diana K. Darnell and Gary C. Schoenwolf. Culture of Avian Embryos, Diana K. Darnell and Gary C. Schoenwolf. Ex Ovo Culture of Avian Embryos, Tamao Ono. Culture of Preimplantation Mouse Embryos, Adam S. Doherty and Richard M. Schultz. In Vitro Culture of Rodent Embryos During Early Postimplantation Period, Masahiko Fujinaga. Cryopreservation of Mouse Embryos, Jean Richa.
Part III. Developmental Pattern and Morphogenesis. Studying Head and Brain Development in Drosophila, Robert Finkelstein. Bioassays of Inductive Interactions in Amphibian Development, Takashi Ariizumi, Kazuhiro Takano, Makoto Asashima, and George M. Malacinski. Gastrulation and Early Mesodermal Patterning in Vertebrates, Gary C. Schoenwolf and Jodi L. Smith. Craniofacial Development of Avian and Rodent Embryos, Brian K. Hall and Tom Miyake. Examination of the Axial Skeleton of Fetal Rodents, Michael G. Narotsky and John M. Rogers. Cardiac Morphogenesis and Dysmorphogenesis: An Immunohistochemical Approach,
B. Rush Waller III and Andy Wessels.
Part IV. Embryo Structure and Function. Application of Plastic Embedding for Sectioning Whole-Mount Immunostained Early Vertebrate Embryos, Kersti K. Linask and Takeshi Tsuda. Confocal Microscopy of Live Xenopus Oocytes, Eggs, and Embryos, Carolyn A. Larabell. Whole-Mount Immunolabeling of Embryos by Microinjection: Increased Detection Levels of Extracellular and Cell Surface Epitopes, Charles D. Little and Christopher J. Drake. Confocal Laser Scanning Microscopy of Morphology and Apoptosis in Organogenesis-Stage Mouse Embryos, Robert M. Zucker, E. Sidney Hunter III, and John M. Rogers. Embryo/Fetal Topographical Analysis by Fluorescence Microscopy and Confocal Laser Scanning Microscopy, Robert M. Zucker and John M. Rogers. Magnetic Resonance Imaging Analysis of Embryos, Bradley R. Smith. Optical Coherence Tomography Imaging in Developmental Biology, Stephen A. Boppart, Mark E. Brezinski, and James G. Fujimoto. Ultrasound Backscatter Microscopy of Mouse Embryos, Daniel H. Turnbull. Use of Doppler Echocardiography to Monitor Embryonic Mouse Heart Function, Kersti K. Linask and James
C. Huhta. Calcium Imaging and Cell-Cell Signaling, Diane C. Slusarski and Victor G. Corces. Acquisition, Display, and Analysis of Digital Three-Dimensional and Time-Lapse (Four-Dimensional) Data Sets Using Free Software Applications, Charles F. Thomas and John G. White.
Part V. Cell Lineage Analysis. Cell Lineage Analysis: Applications of Green Fluorescent Protein, Magdalena Zernicka-Goetz and Jonathon Pines. Cell Lineage Analysis: X-Inactivation Mosaics, Seong-SengTan, Leanne Godinho, and Patrick P. L. Tam. Retroviral Cell Lineage Analysis in the Developing Chick Heart, Robert G. Gourdie, Gang Cheng, Robert P. Thompson, and Takashi Mikawa. Dynamic Labeling Techniques for Fate Mapping, Testing Cell Commitment, and Following Living Cells in Avian Embryos, Diana K. Darnell, Virginio Garcia-Martinez, Carmen Lopez-Sanchez, Shipeng Yuan, and Gary C. Schoenwolf. Cell Lineage Analysis: Videomicroscopy Techniques, Paul J. Heid and Jeff Hardin. Cell Lineage Analysis in Xenopus Embryos, Sally A. Moody. Photoactivatable (Caged) Fluorescein as a Cell Tracer for Fate Mapping in the Zebrafish Embryos, David J. Kozlowski and Eric S. Weinberg. Carboxyfluorescein as a Marker at Both Light and Electron Microscope Levels to Follow Cell Lineage in the Embryo, Dazhong Sun, C. May Griffith, and Elizabeth
D. Hay.
Part VI. Chimeras. Transplantation Chimeras: Use in Analyzing Mechanisms


"We live in a time of rising expectations. Being a developmental biologist at the turn of the millenium demands expertise in embryological manipulation, viral gene expression, in situ hybridization, videomicroscopy, in vitro assays, transgenic embryo production, cell lineage analysis, PCR techniques, and computer-enhanced imaging technology. Rocky Tuan and Cecilia Lo have done the field (and each scientist within it) a wonderful service by collecting and editing the protocols of the masters of each echnique. More than 100 papers spanning these areas (and more) patiently lead one through each method, giving details on what brand of microscope slides to use, what centrifuge tubes work best, and where to purchase each piece of equipment. Copious notes provide details based on the laboratories' experiences of what works and what doesn't. Developmental Biology Protocols is a library in itself and will be essential for every laboratory of developmental biology. This
is the collection to get before your next grant application is due."-Scott Gilbert, Department of Biology, Swarthmore College, Swarthmore, PA
"The range of techniques, from viral vectors to imaging, and of organisms, from sea urchins to mammals, is comprehensive yet focused. This work should be an invaluable aid to those interested in all aspects of comparative development. This work is logically organized and presents a great opportunity for not only picking up a technique but also placing it in a logical context. Well done!" -Barbara B. Knowles, Director of Research, Jackson Labs, Bar Harbor, ME
"Among similar laboratory manuals Developmental Biology Protocols edited by Tuan and Lo impress by their breadth of coverage, timeliness, and scrupulous attention to detail. . .these protocols will be an invaluable aid to every geneticist and developmental biologist."-Davor Solter, Director of Developmental Biology, Max-Planck Institute, Freiburg, Germany
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