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DNA Topoisomerase Protocols: Volume II: Enzymology and Drugs

Volume II: Enzymology and Drugs. 'Methods in Molecular Biology'. Auflage 2001. Sprache: Englisch.
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Beginning with the Escherichia coli ? protein, or bacterial DNA topoisomerase I, an ever-increasing number of enzymes have been identified that catalyze changes in the linkage of DNA strands. DNA topoisomerases are ubiquitous in nature and have been … weiterlesen
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DNA Topoisomerase Protocols: Volume II: Enzymology and Drugs als Buch

Produktdetails

Titel: DNA Topoisomerase Protocols: Volume II: Enzymology and Drugs

ISBN: 0896035123
EAN: 9780896035126
Volume II: Enzymology and Drugs.
'Methods in Molecular Biology'.
Auflage 2001.
Sprache: Englisch.
Herausgegeben von Neil Osheroff, Mary-Ann Bjornsti
SPRINGER VERLAG GMBH

20. Oktober 2000 - gebunden - 334 Seiten

Beschreibung

Beginning with the Escherichia coli ? protein, or bacterial DNA topoisomerase I, an ever-increasing number of enzymes have been identified that catalyze changes in the linkage of DNA strands. DNA topoisomerases are ubiquitous in nature and have been shown to play critical roles in most p- cesses involving DNA, including DNA replication, transcription, and rec- bination. These enzymes further constitute the cellular targets of a number of clinically important antibacterial and anticancer agents. Thus, further studies of DNA topology and DNA topoisomerases are critical to advance our und- standing of the basic biological processes required for cell cycle progression, cell division, genomic stability, and development. In addition, these studies will continue to provide critical insights into the cytotoxic action of drugs that target DNA topoisomerases. Such mechanistic studies have already played an important role in the development and clinical application of antimicrobial and chemotherapeutic agents. The two volumes of DNA Topoisomerase Protocols are designed to help new and established researchers investigate all aspects of DNA topology and the function of these enzymes. The chapters are written by prominent investigators in the field and provide detailed background information and st- by-step experimental protocols. The topics covered in Part I: DNA Topology and Enzymes, range from detailed methods to analyze various aspects of DNA structure, from linking number, knotting/unknotting, site-specific recombi- tion, and decatenation to the overexpression and purification of bacterial and eukaryotic DNA topoisomerases from a variety of cell systems and tissues.

Inhaltsverzeichnis

Assaying DNA Topoisomerase I Relaxation Activity
Lance Stewart and James J. Champoux

DNA Topoisomerase II-Catalyzed DNA Decatenation
Andrea Haldane and Daniel M. Sullivan

Plasmid DNA Supercoiling by DNA Gyrase
Penny J. Sayer, Martin L. Goble, Mark Oram, and L. Mark Fisher

Analyzing Reverse Gyrase Activity
Michel Duguet, Christine Jaxel, Anne-Céile Déclais, Fabrice Confalonieri, Janine Marsault, Claire Bouthier de la Tour, Marc Nadal, and Christiane Portemer

Topoisomerase II-Catalyzed ATP Hydrolosis as Monitored by Thin-Layer Chromatography
Paul S. Kingma, John M. Fortune, and Neil Osheroff

Use of a Real-Time, Coupled Assay to Measure the ATPase Activity of DNA Topoisomerase II
Janet E. Lindsley

Analysis of Topoisomerase-DNA Interactions by Electrophoretic Mobility Shift Assay
Stewart Shuman

Filter Binding Assays for Topoisomerase-DNA Complexes
Joaquim Roca

DNA Topoisomerase I-Mediated Nicking of Circular Duplex DNA
James J. Champoux

Analysis of Cleavage Complexes Using Reactive Inhibitor Derivatives
Kenneth N. Kreuzer, Catherine H. Freudenreich, and Yves Pommier

Uncoupling of Topoisomerase-Mediated DNA Cleavage and Religation
Anni H. Anderson, Kent Christiansen, and Ole Westergaard

Synthesis and Use of DNA Containing a 5'-Bridging Phosphorothioate as a Suicide Substrate for Type I DNA Topoisomerases
Alex B. Burgin, Jr

Isolation of Covalent Enzyme-DNA Complexes
Thomas C. Rowe, Dale Grabowski, and Ram Ganapathi

ICE Bioassay: Isolating In Vivo Complexes of Enzyme to DNA
Deepa Subramanian, Christine Sommer Furbee, and Mark T. Muller

DNA Unwinding Test Using Eukaryotic DNA Topoisomerase I
Linus L. Shen

Drug-DNA Interactions
David E. Graves

Quinolone Interactions with DNA and DNA Gyrase
Linus L.Shen

Bactericidal Assays for Fluoroquinolones
Thomas D. Gootz

Drug Toxicity in E. coli Cells Expressing Human DNA Topoisomerase I
Scott T. Taylor and Rolf Menzel

Drug-Induced Cytotoxicity in Tissue Culture
Cynthia E. Herzog and Leonard A. Zwelling

Analysis of Drug Uptake-Flow Cytometry
Grace Zoorob and Thomas Burke

Cell Cycle Analysis of Drug-Treated Cells
Frank Traganos, Gloria Juan, and Zbigniew Darzynkiewicz

Assaying Drug-Induced Apoptosis
Zbigniew Darzynkiewicz, Gloria Juan, and Frank Traganos

Single-Strand Conformational Polymorphism Analysis of DNA Topoisomerases I and II
Mary K. Danks

PCR-Based Cloning of DNA Topoisomerase Genes
Wai Mun Huang

Topoisomerase II-Catalyzed Relaxation and Catenation of Plasmid DNA
John M. Fortune and Neil Osheroff

Topoisomerase II-Mediated Cleavage of Plasmid DNA
D. Andrew Burden, Stacie J. Froelich-Ammon, and Neil Osheroff

Drug-induced Stabilization of Covalent DNA Topoisomerase I-DNA Intermediates: DNA Cleavage Assays
Paola Fiorani, Christine L. Hann, Piero Benedetti, and Mary-Ann Bjornsti

Studying DNA Topoisomerase I-Targeted Drugs in the Yeast Saccharomyces cerevisiae
Michael H. Woo, John R. Vance, and Mary-Ann Bjornsti

Yeast Systems for Demonstrating the Transfer of Antitopoisomerase II Agents
John L. Nitiss and Karen C. Nitiss

Index

Pressestimmen

"This book completes a comprehensive collection of assays, including the weird and wonderful, that can be used to investigate the enzymology and drug inhibition of topoisomerases...I recommend any students about to undertake a PhD degree in this area to buy a copy. Even those who are well versed in the biology of topoisomerases should consider buying both volumes of this book, they will surely find some things they didn't know - I did!" - MicroBiology Today
"Described by expert experimentalists who have perfected the techniques, these reproducible methods include assays for enzyme-catalyzed DNA relaxation/supercoiling, DNA cleavage, DNA nicking, DNA decatentaion, and ATP hydrolysis." - Anticancer Research
"An unprecedented collection of cutting-edge experimental protocols for investigating the catalytic activities of DNA topoisomerases, as well as their specific interactions with topoisomerase-targeted antitumor and antibacterial drugs." -Tumori

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