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Epstein-Barr Virus Protocols

'Methods in Molecular Biology'. Auflage 2001. Book. Sprache: Englisch.
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The discovery of Epstein-Barr virus (EBV) by Epstein, Achong, and Barr, reported in 1964 (Lancet 1:702-703), was stimulated by Denis Burkitt's rec- nition of a novel African childhood lymphoma and his postulation that an infectious agent was involved … weiterlesen
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Produktdetails

Titel: Epstein-Barr Virus Protocols

ISBN: 0896036901
EAN: 9780896036901
'Methods in Molecular Biology'.
Auflage 2001.
Book.
Sprache: Englisch.
Herausgegeben von Gerhard H. W. May, Joanna B. Wilson
Humana Press

23. März 2001 - gebunden - 456 Seiten

Beschreibung

The discovery of Epstein-Barr virus (EBV) by Epstein, Achong, and Barr, reported in 1964 (Lancet 1:702-703), was stimulated by Denis Burkitt's rec- nition of a novel African childhood lymphoma and his postulation that an infectious agent was involved in the tumor's etiology (Nature194:232-234, 1962). Since then, molecular and cellular biological and computational technologies have progressed by leaps and bounds. The advent of recombinant DNA technology opened the possibilities of genetic research more than most would have realized. Not only have the molecular tools permitted the analyses of viral mechanisms, but, importantly, they have formed the basis for discerning viral presence and, subsequently, viral involvement in an increasing number of diseases. Though in every field of science the search for further knowledge is likely to be a limitless phenomenon, the distinct goal in EBV research, namely, to gain sufficient insight into the viral-host interaction to be able to intercept the pathogenic process, is beginning to be realized. Epstein-Barr virus research has effectively entered the postgenomic era that began with the sequencing of the first strains, cloned in the mid to late 1980s.

Inhaltsverzeichnis

I. Genome and Transcript Analyses

Epstein-Barr Virus: The B95-8 Strain Map
Paul J. Farrell

Analysis of Replication of oriP-Based Plasmids by Quantitative, Competitive PCR
Ann L. Kirchmaier

Genetic Analysis and Gene Expression with Mini-Epstein-Barr Virus Plasmids
Ellen Kilger and Wolfgang Hammerschmidt

Construction of cDNA Libraries for the Analysis of the Structure of Complementary Strand Transcripts (CSTs)

Paul R. Smith. Analysis of the Expression and Function of the EBV-Encoded Small RNAs, the EBERs, in Heterologous Cells
Kenneth G. Laing, Volker Matys, and Michael J. Clemens

Visualizing EBV Expression Patterns by FISH
Anna Szeles

II. Viral Detection

In Situ Detection of Epstein-Barr Virus DNA and Viral Gene Products
Gerald Niedobitek and Hermann Herbst

Phenotype Determination of Epstein-Barr Virus-Infected Cells in Tissue Sections
Hermann Herbst and Gerald Niedobitek

Detection of EBV Infection at the Single-Cell Level: Precise Quantitation of Virus-Infected Cells In Vivo
Gregory J. Babcock, Emily M. Miyashita-Lin, and David A. Thorley-Lawson

Detection and Discrimination of Latent and Replicative Herpesvirus Infection at the Single Cell Level In Vivo
Lisa L. Decker, Gregory J. Babcock, and David A. Thorley-Lawson

III. Culture Methods

Virus Isolation
Lindsey M. Hutt-Fletcher and Susan M. Turk

Generation of Lymphoblastoid Cell Lines (LCLs)
Teresa Frisan, Victor Levitsky, and Maria Masucci

Cell Cycle Distribution of B-Lymphocytes and Cell Lines
Alison J. Sinclair

Introduction of Plasmid Vectors into Cells Via Electroporation
Ann L. Kirchmaier

Malignant Transformation and Immortalization Assays in Animal Cells Transfected with the BARF1 Gene
Tadamasa Ooka

Transient Gene Expression and MACS Enrichment
Dieter Kube and Martina Vockerodt

In Vitro Assays to Study Epithelial Cell Growth
Christopher W. Dawson and Lawrence S. Young

In Vitro Assays to Study Epithelial Cell Differentiation
Christopher W. Dawson, and Lawrence S. Young

Cell Sensitivity Assays: Quantitative Detection of Apoptotic Cells In Vitro Using the TUNEL Assay
Neil A. Jones and Caroline Dive

Qualitative Detection of Apoptotic Cells Assessed by DNA Fragmentation
Amanda J. McIlwrath

IV. Immune Assays

Regression Assay
Teresa Frisan, Victor Levitsky, and Maria Masucci

Generation of Polyclonal EBV-Specific CTL Cultures and Clones
Victor Levitsky, Teresa Frisan, and Maria Masucci

Determination of Antigen and Fine Peptide Specificity of EBV-Specific CTLs
Victor Levitsky, Teresa Frisan, and Maria Masucci

Limiting Dilution Assay
Teresa Frisan, Victor Levitsky, and Maria Masucci

V. Viral Protein Detection

Antibodies for Detecting EBV Latent Proteins
Martin Rowe, and Anja Mehl

Detection of EBV Latent Proteins by Western Blotting
Martin Rowe and Matthew Jones

Biosynthetic Radiolabeling of Virus Glycoproteins for Immunoprecipitation and Electrophoretic Analysis
Lindsey M. Hutt-Fletcher and Susan M. Turk

VI. Protein-Protein, Protein-DNA, and Protein-RNA Interactions

The Yeast Two-Hybrid Assay to Identify Interacting Proteins
Kenneth M. Izumi

Identification of Transactivation, Repression, and Protein-Protein Interaction Domains Using GAL4-Fusion Proteins
James J.-D. Hsieh and S. Diane Hayward

Magnetic DNA Affinity Purification of a Cellular Transcription Factor
Lothar J. Strobl and Ursula Zimber-Strobl

Screening an Expression Library with a DNA Binding Site
Qin Zhang and Eduardo A. Montalvo

Analysis of DNA Binding Proteins by Mobility S

Pressestimmen

"Timely and highly practical, Epstein-Barr Virus Protocols provides powerful tools for elucidating life cycle of EBV and its host interactions, work that promises the emergence of major new treatments and cures of EVB-associated diseases, including several forms of human cancer." - Anticancer Research
"Overall it is an excellent resource aimed at postgraduate students and young researchers. Protocols are extensively referenced to the primary literature at the end of each chapter." -Today's Life Science
"This book provides a well-written and concise account of many of the protocols currently in use for the study of the Epstein-Barr virus and its related diseases. It is comprehensive..." "Both volumes are laid out attractively...and contain much valuable information on the pharmacological aspects of neurotoxicology, as well as state-of-the-art methodology." -Microbiology Today
"Leading-edge and forward-looking, Tumor Suppressor Genes in Human Cancer illuminates what is currently known of tumor suppressor genes and their regulation, work that is already beginning to revolutionize, and treatment design." -Tumori
"This book is highly recommended for collections serving researchers in the area of molecular biology." -E-Streams
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