Titel: Chemical Biology
Autor/en: Herbert Waldmann, Petra Janning
A Practical Course.
101 Abbildungen, davon 1 farbig.
Wiley VCH Verlag GmbH
24. Juni 2004 - kartoniert - 220 Seiten
Take your first steps in combinatorial synthesis or synthesize bioactive molecules such as antibiotics. Search for mutations in DNA using chemical probes or perform a proteome analysis in yeast.
The present selection of 12 inspiring experiments is tailored for maximum learning effect at minimal expense of time and equipment. Almost all currently used laboratory techniques in synthesis and analysis of bioactive compounds are represented at least once.
Abundant practical hints are complemented by a thorough treatment of the underlying theory and mechanisms.
DNA-Synthesis and DNA-Hybridization
Doubly Labeled Peptide Nucleic Acids as Probes for the Detection of DNA Point Mutations
Synthesis and Characterization of a Covalent Oligonucleotide-Streptavidin Conjugate for DNA-directed Immobilization
Solid Phase Synthesis of Peptides: Bradykinin Analogs and the Evaluation of Calcium Mobilization in PC-12 Cells
In silico Protein Ligand Design
Lipidation of Proteins and Peptides - Farnesylation of the Ras protein
Insertion of Lipidated Peptides in Model Membranes
Isolation of Potato Phosphorylase and Enzymatic Synthesis of Amylose
Proteome Analysis - Identification of Proteins Isolated from Yeast
Lectins - Determination of the Sugar Specificity of Jacalin by a Sugar-Lectin Binding Assay
Combinatorial Chemistry - Ugi-Synthesis, Genetic Algorithm, and Determination of Enzyme Inhibition
Solid-phase Synthesis of an Antibiotic
Glossary of Terms
Herbert Waldmann (b 1957) received his PhD in 1985 from the University of Mainz (Germany) with H. Kunz. After postdoctoral studies (1985-1986, Harvard University, George Whitesides) and habilitation (1991, University of Mainz) he was appointed associate professor at the University of Bonn in 1991. In 1993 he moved to the University of Karlsruhe as Full Professor of Organic Chemistry. In 1999 he was appointed Director at the Max Planck Institute of Molecular Physiology in Dortmund, where he is heading the Department of Chemical Biology. He also holds a Professorship in Biochemistry at the University of Dortmund. Herbert Waldmann has been the recipient of the Friedrich Weygand Award for the advancement of peptide chemistry, of the Carl Duisberg Award of the Gesellschaft Deutscher Chemiker, the Otto-Bayer-Award and the Steinhofer Award of the Steinhofer Foundation.
His current research interests include bioorganic chemistry and natural product synthesis as well as biocatalysis, stereoselective synthesis and combinatorial chemistry.
Petra Janning (b. 1967) studied chemistry at the University of Münster (Germany). She completed her PhD degree at the University of Dortmund with G. Tölg and M. Linscheid. From 1995 - 1998 she worked as a chemist in the University Hospital in Aachen. From 1999-2001 she worked in the group of G.H. Degen and H.M. Bolt at the Institute of Occupational Health in Dortmund. In 2001 she joined the Max Planck Institute of Molecular Physiology, where she is currently working in the group of Herbert Waldmann. She is responsible for the Bioorganic Practical Course and the analytics in the department.
"... an exciting and lively book that takes students to the point where they can start to become effective researchers..." Chemistry World
"... a practical, valuable book for faculty since virtually no textbooks exist...will be useful even to those who are not planning laboratory courses." CHOICE
"... a well-written book containing relevant experimental detail and background information ... a useful addition to many libraries." Applied Organometallic Chemistry
"Die Stärke des Buches ist es, konkrete Einzelheiten zu Arbeitstechniken und aktuelle Forschung gleichermaßen zu präsentieren." Henning Breyhan, Thomas Kolter, Kekulé -Institut für Organische Chemie und Biochemie, Universität Bonn, Angewandte Chemie, 4. Januarheft 2005-117/4