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Myeloid Leukemia

Methods and Protocols. 'Methods in Molecular Medicine'. Auflage 2006. Book. Sprache: Englisch.
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A panel of internationally recognized research scientists and clinical investigators brings together a diverse collection of readily reproducible methods for identifying and quantifying a large number of specific genetic abnormalities associated with … weiterlesen
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Titel: Myeloid Leukemia

ISBN: 1588294854
EAN: 9781588294852
Methods and Protocols.
'Methods in Molecular Medicine'.
Auflage 2006.
Book.
Sprache: Englisch.
Herausgegeben von Mark Hertzberg, Harry Iland, Paula Marlton
Humana Press

15. November 2005 - gebunden - 320 Seiten

Beschreibung

A panel of internationally recognized research scientists and clinical investigators brings together a diverse collection of readily reproducible methods for identifying and quantifying a large number of specific genetic abnormalities associated with the broad spectrum of myeloid malignancies. Highlights include techniques for the detection of BCR-ABL mutations and resistance to imatinib mesylate, detection of the FIP1L1-PDGFRA fusion in idiopathic hypereosinophilic syndrome and chronic eosinophilic leukemia, classification of AML by DNA-oligonucleotide microarrays, and detection of the V617F JAK2 mutation in myeloproliferative disorders. In addition to gene rearrangments, other prognostically relevant molecular lesions such as FLT3 mutations and WT-1 overexpression are covered.

Inhaltsverzeichnis

Isolation of RNA and DNA From Leukocytes and cDNA Synthesis
Joop H. Jansen and Bert A. van der Reijden

Cytogenetic and FISH Techniques in Myeloid Malignancies
Lynda J. Campbell

Overview of Real-Time RT-PCR Strategies for Quantification of Gene Rearrangements in the Myeloid Malignancies
Christophe Picard, Monique Silvy, and Jean Gabert

Diagnosis and Monitoring of Chronic Myeloid Leukemia by Qualitative and Quantitative RT-PCR
Susan Branford and Timothy Hughes

Detection of BCR-ABL Mutations and Resistance to Imatinib Mesylate
Susan Branford and Timothy Hughes

Deletion of the Derivative Chromosome 9 in Chronic Myeloid Leukemia
Lynda J. Campbell

Diagnosis and Monitoring of PML-RARA-Positive Acute Promyelocytic Leukemia by Qualitative RT-PCR
Vincenzo Rossi, Laura Levati, and Andrea Biondi

Diagnosis and Monitoring of PML-RARa-Positive Acute Promyelocytic Leukemia by Quantitative RT-PCR
Elisa Mokany, Alison V. Todd, Caroline J. Fuery, and Tanya L. Applegate

Diagnosis and Monitoring of AML1-MTG8 (ETO)-Positive Acute Myeloid Leukemia by Qualitative and Real-Time Quantitative RT-PCR
Khalid Tobal and John A. Liu Yin

Diagnosis and Monitoring of CBFB-MYH11-Positive Acute Myeloid Leukemia by Qualitative and Quantitative RT-PCR
Bert A. van der Reijden and Joop H. Jansen

Detection of the FIP1L1-PDGFRA Fusion in Idiopathic Hypereosinophilic Syndrome and Chronic Eosinophilic Leukemia
Jan Cools, Elizabeth H. Stover, and D. Gary Gilliland

FLT3 Mutations in Acute Myeloid Leukemia
Hitoshi Kiyoi and Tomoki Naoe

WT-1 Overexpression in Acute Myeloid Leukemia and Myelodysplastic Syndromes
Daniela Cilloni, Enrico Gottardi, and Giuseppe Saglio

Classification of AML by DNA-Oligonucleotide Microarrays
Alexander Kohlmann, Wolfgang Kern, Wolfgang Hiddemann, and Torsten Haferlach

Classification of AML Using a Monoclonal Antibody Microarray
Richard I. Christopherson, Kerryn Stoner, Nicole Barber, Larissa Belov, Adrian Woolfson, Mike Scott, Linda Bendall, and Stephen P. Mulligan

Methods for the Detection of the JAK2 V617F Mutation in Human Myeloproliferative Disorders
Peter J. Campbell, Linda M. Scott, E. Joanna Baxter, Anthony J. Bench, Anthony R. Green, and Wendy N. Erber

Overexpression of PRV-1 Gene in Polycythemia Rubra Vera and Essential Thrombocythemia
Maurizio Martini, Luciana Teofili, and Luigi M. Larocca

Chimerism Analysis Following Nonmyeloablative Stem Cell Transplantation
Thomas Lion and Franz Watzinger

Index
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