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NanoBiotechnology Protocols

'Methods in Molecular Biology'. Auflage 2005. Book with CD-ROM. Sprache: Englisch.
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Hands-on experts in nanomaterial synthesis and application describe in detail the key experimental techniques currently employed in novel materials synthesis, dynamic cellular imaging, and biological assays. The author's emphasize diverse strategies … weiterlesen
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Titel: NanoBiotechnology Protocols

ISBN: 1588292762
EAN: 9781588292766
'Methods in Molecular Biology'.
Auflage 2005.
Book with CD-ROM.
Sprache: Englisch.
Herausgegeben von Sandra J Rosenthal, David Wright
Humana Press

27. April 2005 - gebunden - 244 Seiten

Beschreibung

The combination of nanoscience and biotechnology promises to yield revolutionary biological insights, ranging from receptor function to drug discovery to personal medicine. In NanoBiotechnology Protocols, hands-on experts in nanomaterial synthesis and application describe in detail the key experimental techniques currently employed in novel materials synthesis, dynamic cellular imaging, and biological assays. The authors emphasize diverse strategies to synthesize and functionalize the use of nanoparticles for biological applications. Additional chapters focus on the use of biological components (peptides, antibodies, and DNA) to synthesize and organize nanoparticles to be used as a building block in larger assemblies. These new materials make it possible to image cellular processes for longer durations, leading to high throughput cellular-based screens for drug discovery, drug delivery, and diagnostic applications. Highlights include overview chapters on quantum dots and DNA nanotechnology, and cutting-edge techniques in the emerging nanobiotechnology arena. A value-added compact disk containing color figures is included. The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Illuminating and cross-disciplinary, NanoBiotechnology Protocols enables novice and experienced researchers alike to quickly come up to speed with both nanomaterials preparation and the use of nanomaterials in biological and medicinal applications.

Inhaltsverzeichnis

Applications of Quantum Dots in Biology: An Overview
Charles Z. Hotz

Fluoroimmunoassays Using Antibody-Conjugated Quantum Dots
Ellen R. Goldman, Hedi Mattoussi, George P. Anderson, Igor L. Medintz, and J. Matthew Mauro

Labeling Cell-Surface Proteins Via Antibody Quantum Dot Streptavidin Conjugates
John N. Mason, Ian D. Tomlinson, Sandra J. Rosenthal, and Randy D. Blakely

Peptide-Conjugated Quantum Dots: Imaging the Angiotensin Type 1 Receptor in Living Cells
Ian D. Tomlinson, John N. Mason, Randy D. Blakely, and Sandra J. Rosenthal

Quantum Dot-Encoded Beads
Xiaohu Gao and Shuming Nie

Use of Nanobarcodes® Particles in Bioassays
R. Griffith Freeman, Paul A. Raju, Scott M. Norton, Ian D. Walton, Patrick C. Smith, Lin He, Michael J. Natan, Michael Y. Sha, and Sharron G. Penn

Assembly and Characterization of Biomolecule-Gold Nanoparticle Conjugates and Their Use in Intracellular Imaging
Alexander Tkachenko, Huan Xie, Stefan Franzen, and Daniel L. Feldheim

Whole-Blood Immunoassay Facilitated by Gold Nanoshell-Conjugate Antibodies
Lee R. Hirsch, Naomi J. Halas, and Jennifer L. West

Assays for Selection of Single-Chain Fragment Variable Recombinant Antibodies to Metal Nanoclusters
Jennifer Edl, Ray Mernaugh, and David W. Wright

Surface-Functionalized Nanoparticles for Controlled Drug Delivery
Sung-Wook Choi, Woo-Sik Kim, and Jung-Hyun Kim

Screening of Combinatorial Peptide Libraries for Nanocluster Synthesis
Joseph M. Slocik and David W. Wright

Structural DNA Nanotechnology: An Overview
Nadrian C. Seeman

Nanostructured DNA Templates
Jeffery L. Coffer, Russell F. Pinizzotto, and Young Gyu Rho

Probing DNA Structure With Nanoparticles
Rahina Mahtab and Catherine J. Murphy

Synthetic Nanoscale Elements for Delivery of Materials Into Viable Cells
TimothyE. McKnight, Anatoli V. Melechko, Michael A. Guillorn, Vladimir I. Merkulov, Douglas H. Lowndes, and Michael L. Simpson

Real-Time Cell Dynamics With a Multianalyte Physiometer
Sven E. Eklund, Eugene Kozlov, Dale E. Taylor, Franz Baudenbacher, and David E. Cliffel

Index
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