Targeted at beginners as well as experienced users, this handy reference explains the benefits and uses of flow cytometery in the study of plants and their genomes. Following a brief introduction that highlights general considerations when analyzing plant cells by flow cytometric methods, the book goes on to discuss examples of application in plant genetics, genomic analysis, cell cycle analysis, marine organism analysis and breeding studies.
With its list of general reading and a glossary of terms, this first reference on FCM in plants fills a real gap by providing first-hand practical hints for the growing community of plant geneticists.
Inhaltsverzeichnis
Cytometry and Cytometers: Development and Growth (H. Shapiro)
Principles of Flow Cytometry (J. Robinson, G. Gregori)
Flow Cytometry with Plants: an Overview (J. Dolezel, J. Greilhuber, J. Suda)
Nuclear DNA Content Measurement (J. Greilhuber, E. Temsch, J. Loureiro)
Flow Cytometry and Ploidy: Applications in Plant Systematics, Ecology and Evolutionary Biology (J. Suda, P. Kron, B. Husband, P. Travnicek)
Reproduction Mode Screening (F. Matzk)
Genome Size and its Uses: the Impact of Flow Cytometry (I. Leitch, M. Bennett)
DNA Base Composition of Plant Genomes (A. Meister, M. Barow)
Detection and Viability Assessment of Plant Pathogenic Microorganisms using Flow Cytometry (J. Bergervoet, J. van der Wolf, J. Peters)
Protoplast Analysis using Flow Cytometry and Sorting (D. Galbraith)
Flow Cytometry of Chloroplasts (E. Pfundel, A. Meister)
DNA Flow Cytometry in Non-vascular Plants (H. Voglmayr)
Phytoplankton and their Analysis by Flow Cytometry (G. Dubelaar, R. Casotti, G. Tarran, I. Biegala)
Cell Cycle Analysis in Plants (M. Pfosser, Z. Magyar, L. Bogre)
Endopolyploidy in Plants and its Analysis by Flow Cytometry (M. Barow, G. Jovtchev)
Chromosome Analysis and Sorting (J. Dolezel, M. Kubalakova, P. Suchankova, P. Kovarova, J. Bartos, H. Simkova)
Analysis of Plant Gene Expression Using Flow Cytometry and Sorting (D. Galbraith)
Flower: A Plant DNA Flow Cytometry Database (J. Loureiro, J. Suda, J. Dolezel, C. Santos)
