Glycosaminoglycans

Glycosaminoglycans: Chemistry and Biology emphasizes several areas of glycosaminoglycan research especially analysis and application of GAGs using a variety of approaches. Chapters focus on chemical and enzymatic synthesis of GAGs for therapeutic purposes; biophysical and biochemical methods for studying GAG-protein interactions; molecular approaches for modulating and defining GAG biosynthesis; informatics approaches for deciphering GAG code; computational approaches for establishing specific and/or non-specific interactions and genetic and biochemical tools for manipulating/visualizing glycosaminoglycan expression and studying their functions in a variety of model organisms. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Glycosaminoglycans: Chemistry and Biology serves as a valuable manual for cutting edge methodologies and practical tips to overcome any obstacles with experimentation pertaining to chemistry and biology of glycosaminoglycans.

Automated Synthesis Of Chondroitin Sulfate Oligosaccharides. - Enzymatic Synthesis Of Heparan Sulfate And Heparin. - Production Of Size-Defined Heparosan, Heparan Sulfate, and Heparin Oligosaccharides By Enzymatic Depolymerization. - Chemical Modification Of Heparin And Heparosan. - Synthesis Of Sulfur Isotope Labeled Sulfate Donor, 3 -Phosphoadenosine-5 -Phosphosulfate, For Studying Glycosaminoglycan Functions. - Preparation Of Isotope Enriched Heparan Sulfate Precursors For Structural Biology Studies. - Synthesis Of Glycosaminoglycan Mimetics Through Sulfation Of Polyphenols. - Synthesis Of Selective Inhibitors Of Heparan Sulfate And Chondroitin Sulfate Proteoglycan Biosynthesis. - Ascidian (Chordata-Tunicata) Glycosaminoglycans: Extraction, Purification, Biochemical and Spectroscopic Analysis. - Human Blood Glycosaminoglycans: Isolation and Analysis. - Chromatographic Molecular Weight Measurements For Heparin, Its Fragments And Fractions, and Other Glycosaminoglycans. - Mass Spectrometric Methods For The Analysis Of Heparin And Heparan Sulfate. - Validated Capillary Electrophoretic Assays For Disaccharide Composition Analysis Of Galactosaminoglycans In Biologic Samples and Drugs/Neutraceuticals. - Fast Screening Of Glycosaminoglycan Disaccharides By Fluorophore Assisted Carbohydrate Electrophoresis (Face): Applications To Biologic Samples And Pharmaceutical Formulations. - Capillary Electrophoretic Analysis Of Isolated Sulfated Polysaccharides To Characterize Pharmaceutical Products. - Methods For Measuring Exchangeable Protons In Glycosaminoglycans. - Heparan Sulfate Structure: Methods To Study N-Sulfation and Ndst Action. - Analysis Of Hyaluronan Synthase Activity. - A Rapid, Non-Radioactive Assay For Measuring Heparan Sulfate C-5 Epimerase Activity Using. - Aggrecan - Approaches To Study Biophysical And Biomechanical Properties. - Use Of Flow Cytometry For Characterisation And Fractionation Of Cell Populations Based On Their Expression Of Heparan SulfateEpitopes. - A Transgenic Approach To Live Imaging Of Heparan Sulfate Modification Patterns. - Informatics Tools To Advance The Biology Of Glycosaminoglycans and Proteoglycans. - Designing `High-Affinity, High-Specificity Glycosaminoglycan Sequences Through Computerized. - Using Isothermal Titration Calorimetry To Determine Thermodynamic Parameters Of Protein-Glycosaminoglycan Interactions. - Characterizing Protein-Glycosaminoglycan Interactions Using Solution Nmr Spectroscopy. 0- Glycosaminoglycan Protein Interaction Studies Using Fluorescence Spectroscopy. - Studying Glycosaminoglycan Protein Interactions Using Capillary Electrophoresis. - Histochemical Analysis Of Heparan Sulfate 3-O-Sulfotransferase Expression In Mouse Brain. - Keratan Sulfate: Biosynthesis, Structures And Biological Functions. - The Sulfs: Expression, Purification And Substrate Specificity. - The Detection Of Glycosaminoglycans In Pancreatic Islets And Lymphoid Tissues. - Non-Radioactive Glycosyltransferase And Sulfotransferase Assay To Study Glycosaminoglycan Biosynthesis. - Mapping Proteoglycan Functions With Glycosidases. - Cell Substrate Patterning With Glycosaminoglycans To Study Their Biological Roles In The Central. - Analyzing The Role Of Heparan Sulfate Proteoglycans In Axon Guidance In Vivo In Zebrafish. - Murine Models In The Evaluation Of Heparan Sulfate-Based Anticoagulants. - Genetic Approaches In The Study Of Heparan Sulfate Functions In Drosophila. - Measuring Sulfatase Expression And Invasion In Glioblastoma. - Synthesis And Biomedical Applications Of Xylosides. - A Strategic Approach To Identification Of Selective Inhibitors Of Cancer Stem Cells. - Analysis Of The Heavy Chain Modification And Tsg-6 Activity In Pathological Hyaluronan Matrices. - Heparan Sulfate Modulates Slit3-Induced Endothelial Cell Migration. - Glycosaminoglycan Functionalized Nanoparticles Exploit Glycosaminoglycan Functions. - Role Of Glycosaminoglycans In Infectious Disease. - Isolation And Purification OfVersican And Analysis Of Versican Proteolysis. - Analysis Of Human Hyaluronan Synthase Gene Transcriptional Regulation and Downstream Hyaluronan Cell Surface Receptor Mobility In Myofibroblast Differentiation.