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Channels and transporters are multi-functional proteins that mediate substrate transport and signal transmission and simultaneously act as regulators for other proteins and biosensors for environmental materials. Patch clamping is an epoch-making technique that allows researchers to perform real-time measurements of electrogenic channel/transporter functions at the single/multiple molecular level. This book describes not only the conventional patch clamp techniques but also their newly developed variations or applications, such as perforated patch, slice patch, blind patch, in vivo patch, imaging patch, smart patch, and automated patch clamping. These patch clamp techniques are now essential and are extensively used across the life sciences and in related industries. With plain and practical descriptions of patch clamping and how to carry it out, especially for beginners, the book also shows how widely and exquisitely the patch clamp techniques can be applied by expert electrophysiologists. This work serves as a useful guide for young researchers and students in training and laboratory courses as well as for senior researchers who wish to extend their repertoire of techniques.
Inhaltsverzeichnis
Ch. 1 Prologue: The Ion Channel
Ch. 2 Patch-Clamp Techniques: General Remarks
Ch. 3 Whole-Cell Patch Method
Ch. 4 Perforated Whole-Cell Patch-Clamp Technique: A User s Guide
Ch. 5 Methods for Processing and Analyzing Single-Channel Data
Ch. 6 Channel Noise
Ch. 7 Slice Patch Clamp
Ch. 8 Patch-Clamp Recording Method in Slices for Studying Presynaptic Mechanisms
Ch. 9 Analysis of Synaptic Plasticity with the Slice Patch-Clamp Recording Technique
Ch. 10 Patch-Clamp Recordings from Neuronal Dendrites
Ch. 11 In Vivo Blind Patch-Clamp Recording Technique
Ch. 12 Two-Photon Targeted Patch-Clamp Recordings in Vivo
Ch. 13 Transporter Current Measurements
Ch. 14 Giant Patch and Macro Patch
Ch. 15 Pipette Perfusion Technique
Ch. 16 Planar Lipid Bilayer Method for Studying Channel Molecules
Ch. 17 Patch-Clamp Capacitance Measurements
Ch. 18 Brief Guide to Patch-Clamp Current Measurements in Organelle Membranes
Ch. 19 Role of Ion Channels in Plants
Ch. 20 Automated Patch Clamping
Ch. 21 Patch-Clamp Biosensor Method
Ch. 22 Temperature-Evoked Channel Activation: Simultaneous Detection of Ionic Currents and Temperature
Ch. 23 Heterologous Expression Systems and Analyses of Ion Channels
Ch. 24 Patch-Clamp and Single-Cell Reverse Transcription-Polymerase Chain Reaction/Microarray Analysis
Ch. 25 Smart-Patch Technique
Ch. 26 Ion Channel Pore Sizing in Patch-Clamp Experiments
Ch. 27 Digital Recordings of Patch-Clamp Data
Ch. 28 Solutions for Patch-Clamp Experiments
Produktdetails
Erscheinungsdatum
27. April 2012
Sprache
englisch
Auflage
2012
Seitenanzahl
452
Reihe
Springer Protocols Handbooks
Herausgegeben von
Yasunobu Okada
Verlag/Hersteller
Produktart
gebunden
Abbildungen
XI, 439 p.
Gewicht
1041 g
Größe (L/B/H)
260/183/29 mm
ISBN
9784431539926
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From the reviews:
This book is the most current single `how to manual and text on Patch clamp techniques. The whole cell, inside out patches, and membrane techniques are well illustrated. The patch techniques for brain and spinal slices in culture has several chapters in this book. I recommend this to all those with an interest in biochemistry, membrane biology, neurophysiology, neurologists and neurosurgeons. (Joseph J. Grenier, Amazon. com, May, 2014)
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