The CRISPR/Cas Tool Kit for Genome Editing

This book discusses CRISPR/Cas- one of the most powerful tools available to scientists for genome editing. CRISPR/Cas is not only a genome editing tool, but researchers have also engineered it for gene regulation, genome imaging, base editing and epigenome regulations. This book describes the entire toolkit for CRISPR/Cas. The opening section gives an introduction to the technique and compares it with other genome editing tools. Further section gives a historical perspective of the tool, along with its detailed classification. The next chapters describe bioinformatic tools in CRISPR/Cas, and delivery methods for CRISPR/Cas. The book also discusses about the applications of CRISPR/Cas beyond genome editing and use of CRISPR for rewriting genetic codes. The book dedicates a section to the use of CRISPR in plants. The book culminates with a chapter on the current status, challenges and shortcomings of the CRISPR/Cas genome editing tool.
The book would be highly interesting to students and researchers in molecular biology, biochemistry, biotechnology, food science, agriculture and plant sciences.

Chapter 1: An introduction to CRISPR/Cas based genome editing. - Chapter 2: History and classification of CRISPR/Cas systems. - Chapter 3: Bioinformatic tools. - Chapter 4: Delivery methods in CRISPR/Cas platform. - Chapter 5: Re-engineering of CRISPR/Cas system. - Chapter 6: Beyond genome editing: CRISPR approaches. - Chapter 7: RNA editing with CRISPR/Cas13. - Chapter 8: CRISPR for rewriting genetic codes. - Chapter 9: Applications of CRISPR/Cas system in plants. - Chapter 10: Challenges and Future perspective of CRISPR Technology.