The Golgi Complex

4D Confocal Imaging of Yeast Organelles. - Imaging the Polarized Sorting of Proteins from the Golgi Complex in Live Neurons. - Imaging Golgi Outposts in Fixed and Living Neurons. - Analysis of Arf1 GTPase-Dependent Membrane Binding and Remodeling Using the Exomer Secretory Vesicle Cargo Adaptor. - STEM Tomography Imaging of Hypertrophied Golgi Stacks in Mucilage-Secreting Cells. - Reconstitution of COPI Vesicle and Tubule Formation. - Reconstitution of Phospholipase A₂-Dependent Golgi Membrane Tubules. - Proteomic Characterization of Golgi Membranes Enriched from Arabidopsis Suspension Cell Cultures. - High Content Analysis of the Golgi Complex by Correlative Screening Microscopy. - Activity Detection of GalNAc Transferases by Protein-Based Fluorescence Sensors In Vivo. - In Situ Proximity Ligation Assay (PLA) Analysis of Protein Complexes Formed Between Golgi-Resident, Glycosylation-Related Transporters and Transferases in AdherentMammalian Cell Cultures. - Creating Knock-Outs of Conserved Oligomeric Golgi Complex Subunits Using CRISPR-Mediated Gene Editing Paired with a Selection Strategy Based on Glycosylation Defects Associated with Impaired COG Complex Function. - Reversible Controlled Aggregation of Golgi Resident Enzymes to Assess Their Transport/Dynamics Along the Secretory Pathway. - Assays to Study the Fragmentation of the Golgi Complex During the G2/M Transition of the Cell Cycle. - The Role of Lysophospholipid Acyltransferases in the Golgi Complex. - Methods to Purify and Assay Secretory Pathway Kinases.