This volume covers the latest protocols used to study the molecular biology of morbilliviruses, including cell entry, replication, and virus-host interactions. The chapters in this book also cover methods for studying morbillivirus infection through the use of novel tissue culture and in vivo models; analyzing the biological implications of infection by using primary tissues isolated from infected animals; and exploring diagnostic tools to evaluate humoral immunity against human-pathogenic measles virus (MeV). Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, Measles and Related Morbilliviruses: Methods and Protocols is a valuable resource for researchers studying MeV, and will be useful to researchers studying other members of Paramyxoviridae and Mononegavirales.
Inhaltsverzeichnis
A Quantitative Fusion Assay to Study Measles Virus Entry. - A Semi-Quantitative Protein-Fragment Complementation Assay to Study Protein-Protein Interactions of the Polymerase Complex In Cellula. - Design and Execution of In Vitro Polymerase Assays for Measles Virus and Related Mononegaviruses. - Optical Control of Mononegavirus Gene Expression and Replication. - Generation of Defective Interfering Particles of Morbilliviruses Using Reverse Genetics. - Molecular Analysis of Copy Back Defective Interfering RNAs of Morbilliviruses. - Applying Reverse Genetics to Study Measles Virus Interactions with the Host. - Identification of Host Factors of Paramyxoviruses by siRNA Genome-Wide Screens. - Dual RNA-seq Analysis of Patients' Cells and Viral Genome After Measles Infection. - Investigating Liquid-Liquid Phase Separation in Virus-Generated Inclusion Bodies Using Fluorescence Recovery After Photobleaching of Fluorescently Labeled Host Proteins. - Culturing Immortalized Human Airway Epithelial Cells at an Air-Liquid Interface for Measles Virus Infection. - In Vitro Modeling of Feline Morbillivirus Infections Using Primary Feline Kidney Cells. - Setup of Aerosol Delivery System to Prevent Measles Virus Infection in Nonhuman Primate Model. - Histopathological Analysis of Brains from Dogs Infected with Canine Distemper Virus. - Canine Distemper Virus Pathogenesis in the Ferret Model. - Measles Foci Reduction Neutralization Test (FRNT). - Multiplex Bead Assay for Serological Surveillance of Measles and Rubella. - Measles IgG Avidity Assay.
