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Combinatorial Peptide Library Protocols

'Methods in Molecular Biology'. 1998. Auflage. Book. Sprache: Englisch.
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During the course of evolution, an imbalance was created between the rate of vertebrate genetic adaptation and that of the lower forms of living organisms, such as bacteria and viruses. This imbalance has given the latter the advantage of generating, … weiterlesen


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Combinatorial Peptide Library Protocols als Buch


Titel: Combinatorial Peptide Library Protocols

ISBN: 0896033929
EAN: 9780896033924
'Methods in Molecular Biology'.
1998. Auflage.
Sprache: Englisch.
Herausgegeben von Shmuel Cabilly
Humana Press

23. Dezember 1997 - gebunden - 332 Seiten


During the course of evolution, an imbalance was created between the rate of vertebrate genetic adaptation and that of the lower forms of living organisms, such as bacteria and viruses. This imbalance has given the latter the advantage of generating, relatively quickly, molecules with unexpected structures and features that carry a threat to vertebrates. To compensate for their weakness, vertebrates have accelerated their own evolutionary processes, not at the level of whole organism, but in specialized cells containing the genes that code for antibody molecules or for T-cell receptors. That is, when an immediate requirement for molecules capable of specific interactions arose, nature has preferred to speed up the mode of Darwinian evolution in pref- ence to any other approach (such as the use of X-ray diffraction studies and computergraphic analysis). Recently, Darwinian rules have been adapted for test tube research, and the concept of selecting molecules having particular characteristics from r- dom pools has been realized in the form of various chemical and biological combinatorial libraries. While working with these libraries, we noticed the interesting fact that when combinatorial libraries of oligopeptides were allowed to interact with different selector proteins, only the actual binding sites of these proteins showed binding properties, whereas the rest of the p- tein surface seemed "inert. " This seemingly common feature of protein- having no extra potential binding sites--was probably selected during evolution in order to minimize nonspecific interactions with the surrounding milieu.


Synthesis of a One-Bead One-Compound Combinatorial Peptide Library Kit S. Lam and Michal Lebl. Enzyme-Linked Colorimetric Screening of a One-Bead One-Compound Combinatorial Library Kit S. Lam. Synthesis and Screening of Positional Scanning Combinatorial Libraries Colette T. Dooley and Richard
A. Houghten. Synthesis and Screening of Peptide Libraries on Continuous Cellulose Membrane Supports Achim Kramer and Jens Schneider-Mergener. Peralkylation: 'Libraries from Libraries': Chemical Transformation of Synthetic Combinatorial Libraries John M. Ostresh, Barbara Dörner, and Richard A. Houghten . Introduction to Combinatorial Solid-Phase Assays for Enzyme Activity and Inhibition Morten Meldal. Preparation of Biocompatible Resins for Library Syntheses Morten Meldal. Intramolecular Fluorescence-Quenched Substrate Libraries Morten Meldal. The Solid-Phase Enzyme Inhibitor Library Assay Morten Meldal. Determination of Peptide Substrate Motifs for Protein Kinases Using a 'One-Bead One Compound' Combinatorial Library Approach Kit S. Lam. The Use of Peptide Library for the Determination of Kinase Peptide Substrates Zhou Songyang and Lewis C. Cantley. Analysis of Protein Kinase Substrate Specificity by the Use of Peptide Libraries on Cellulose Paper (SPOT-Method) Werner J. Tegge and Ronald Frank. Generation of Multiuse Peptide Libraries for Functional Screenings Channa K. Jayawickreme, Shiranthi P. Jayawickreme, and Michael R. Lerner. Functional Screening of Multiuse Peptide Libraries Using Melanophore Bioassay Channa K. Jayawickreme, Shiranthi P. Jayawickreme, and Michael R. Lerner. The Basic Structure of Filamentous Phage and Its Use in the Display of Combinatorial Peptide Libraries Shmuel Cabilly. Construction and Use of a 20-mer Phage Display Epitope LibraryBaruch Stern and Jonathan M. Gershoni. Construction of Disulfide-Constrained Random Peptide Libraries Displayed on Phage Coat Protein VIII Alessandra Luzzago and Franco Felici . Conformational MimicryThrough Random Constraints Plus Affinity Selection Guangming Zhong. The Use of Combinatorial Libraries to Identify Ligands That Interact with Surface Receptors in Living Cells Shmuel Cabilly, Judith Heldman, Eliahu Heldman, and Ephraim Katchalski-Katzir. Screening Phage Display Peptide Libraries on Nitrocellulose Membranes Shmuel Cabilly, Judith Heldman, and Ephraim Katchalski-Katzir. Identification of Disease-Specific Epitopes Atonella Folgori, Alessandra Luzzago, Paolo Monaci, Alfredo Nicosia, Riccardo Cortese, and Franco Felici. Identification of Peptide Ligands for the Antigen Binding Receptor Expressed on Human B-Cell Lymphomas Markus F. Renschler, William J. Dower, and Ronald Levy. Major Histocompatibility Complex Allele-Specific Peptide Libraries and Identification of T-Cell Mimotopes Marc A. Gavin and Michael J. Bevan. Phage Display of Peptide Libraries on Protein Scaffolds Henry
B. Lowman. Displaying Libraries on Conformationally Constrained Peptides on the Surface of Escherichia coli as Flagellin Fusions Zhijian Lu, Brian
C. Tripp, and John M. McCoy. Combinatorial Peptide Library as an Immunogen J. Estaquier, J.-C. Ameisen, C. Auriault, C. Boutillon, H. Gras-Masse, and A. Tartar. Index.
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