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DNA Repair Protocols

'Methods in Molecular Biology'. Auflage 2006. Sprache: Englisch.
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The first edition of this book, published in 1999 and called DNA Repair Protocols: Eukaryotic Systems, brought together laboratory-based methods for studying DNA damage and repair in diverse eukaryotes: namely, two kinds of yeast, a nematode, a fruit … weiterlesen
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Titel: DNA Repair Protocols

ISBN: 1588295133
EAN: 9781588295132
'Methods in Molecular Biology'.
Auflage 2006.
Sprache: Englisch.
Herausgegeben von Daryl S. Henderson

1. Oktober 2005 - gebunden - 498 Seiten


The first edition of this book, published in 1999 and called DNA Repair Protocols: Eukaryotic Systems, brought together laboratory-based methods for studying DNA damage and repair in diverse eukaryotes: namely, two kinds of yeast, a nematode, a fruit fly, a toad, three different plants, and human and murine cells. This second edition of DNA Repair Protocols covers mammalian cells only and hence its new subtitle, Mammalian Systems. There are two reasons for this fresh emphasis, both of them pragmatic: to cater to the interests of what is now a largely mammalocentric DNA repair field, and to expedite editing and prod- tion of this volume. Although DNA Repair Protocols: Mammalian Systems is a smaller book than its predecessor, it actually contains a greater variety of methods. Fourteen of the book's thirty-two chapters are entirely new and areas of redundancy present in the first edition have been eliminated here (for example, now just two chapters describe assays for nucleotide excision repair [NER], rather than seven). All eighteen returning chapters have been revised, many of them ext- sively. In order to maintain a coherent arrangement of topics, the four-part p- titioning seen in the first edition was dispensed with and chapters concerned with ionizing radiation damage and DNA strand breakage and repair were re- cated to near the front of the book. Finally, an abstract now heads each chapter.


Isolation of Mutagen-Sensitive Chinese Hamster Cell Lines by Replica Plating
Malgorzata Z. Zdzienicka

Complementation Assays Adapted for DNA Repair-Deficient Keratinocytes
Mathilde Fréchet, Valérie Bergoglio, Odile Chevallier-Lagente, Alain Sarasin, and Thierry Magnaldo

Cytogenetic Challenge Assays for Assessment of DNA Repair Capacities
William W. Au and Salama A. Salama

Evaluating the Delayed Effects of Cellular Exposure to Ionizing Radiation
Shruti Nagar, James J. Corcoran, and William F. Morgan

Inhibition of DNA Synthesis by Ionizing Radiation: A Marker for an S-Phase Checkpoint
Nicolaas G. J. Jaspers and Malgorzata Z. Zdzienicka

Analysis of Inhibition of DNA Replication in Irradiated Cells Using the SV40-Based In Vitro Assay of DNA Replication
George Iliakis, Ya Wang, and Hong Yan Wang

Cytometric Assessment of Histone H2AX Phosphorylation: A Reporter of DNA Damage
Xuan Huang and Zbigniew Darzynkiewicz

Detection of DNA Strand Breaks by Flow and Laser Scanning Cytometry in Studies of Apoptosis and Cell Proliferation (DNA Replication)
Zbigniew Darzynkiewicz, Xuan Huang, and Masaki Okafugi

In Vitro Rejoining of Double-Strand Breaks in Genomic DNA
George Iliakis and Nge Cheong

Detection of DNA Double-Strand Breaks and Chromosome Translocations Using Ligation-Mediated PCR and Inverse PCR
Michael J. Villalobos, Christopher J. Betti, and Andrew T. M Vaughan

Plasmid-Based Assays for DNA End-Joining In Vitro
George Iliakis, Bustanur Rosidi, Minli Wang, and Huichen Wang

Use of Gene Targeting to Study Recombination in Mammalian Cell DNA Repair Mutants
Rodney S. Nairn and Gerald M. Adair

Gene-Specific and Mitochondrial Repair of Oxidative DNA Damage
R. Michael Anson, Penelope A. Mason, and Vilhelm A. Bohr

Quantitative PCR-Based Measurement of Nuclear and MitochondrialDNA Damage and Repair in Mammalian Cells
Janine H. Santos, Joel N. Meyer, Bhaskar S. Mandavilli, and Bennett Van Houten

Measuring the Formation and Repair of DNA Damage by Ligation-Mediated PCR
Gerd P. Pfeifer

Immunochemical Detection of UV-Induced DNA Damage and Repair
Marcus S. Cooke and Alistair Robson

A Dot-Blot Immunoassay for Measuring Repair of Ultraviolet Photoproducts
Shirley McCready

Quantification of Photoproducts in Mammalian Cell DNA Using Radioimmunoassay
David L. Mitchell

DNA Damage Quantitation by Alkaline Gel Electrophoresis
Betsy M. Sutherland, Paula V. Bennett, and John C. Sutherland

The Comet Assay: A Sensitive Genotoxicity Test for the Detection of DNA Damage and Repair
Günter Speit and Andreas Hartmann

Fast Micromethod DNA Single-Strand-Break Assay
Heinz C. Schröder, Renato Batel, Heiko Schwertner, Oleksandra Boreiko, and Werner E. G. Müller

32P-Postlabeling DNA Damage Assays: PAGE, TLC, and HPLC
Shinya Shibutani, Sung Yeon Kim, and Naomi Suzuki

Electrophoretic Mobility Shift Assays to Study Protein Binding to Damaged DNA
Vaughn Smider, Byung Joon Hwang, and Gilbert Chu

Construction of MMR Plasmid Substrates and Analysis of MMR Error Correction and Excision
Huixian Wang and John B. Hays

Characterization of Enzymes that Initiate Base Excision Repair at Abasic Sites
Walter A. Deutsch and Vijay Hegde

Base Excision Repair in Mammalian Cells
Yoshihiro Matsumoto

In Vitro Base Excision Repair Assay Using Mammalian Cell Extracts
Guido Frosina, Enrico Cappelli, Monica Ropolo, Paola Fortini, Barbara Pascucci, and Eugenia Dogliotti

Biochemical Assays for the Characterization of DNA Helicases
Robert M. Brosh, Jr. and Sudha Sharma

Repair Synthesis Assay for Nucleotide Excision Repair Activity Using Fractionated Cell


"...It will serve as an essential reference for both the practical and theoretical aspects of DNA repair studies, encourage the transfer of methodologies between model systems, and stimulate the development of new approaches." - Anticancer Research
"...must for any science library supporting a molecular biology program." - E-Streams
"An informative collection of techniques and protocols for characterizing and utilizing DNA repair activities and enzymes from some of the leading names in DNA repair field. This collection of protocols is ideal for both the novice in the field and to the experienced applied researcher." - Lavoisier Librairie
"...an extremely useful resource for any laboratory with an interest in the vibrant field of DNA repair. Any good laboratory manueal should be not only a useful reference guide but also a source of inspiration, and it is undoubtedly true that DNA Repair Protocols: Mammalian Systems succeeds on both counts." -Int. J. Radiation Oncology Biol. Phys.
"This is the definite book on the subject not only for those working in the field of DNA repair but also for those interested in genetic toxicology and chemical safety." -BTS Newsletter

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