A prime reference volume for geneticists, food technologists and biotechnologists in the academic and industrial sectors. Fermentations with lactic acid bacteria determine important qualities such as taste, shelf-life, and food values. New methods of food production require fast and reliable manufacture, which has led to a dramatic surge of interest in the genetic, microbiological and biochemical properties of lactic acid bacteria.
Inhaltsverzeichnis
1 Gene transfer systems and transposition. - 1. 1 Introduction. - 1. 2 Conjugation. - 1. 3 Transduction. - 1. 4 Transformation. - 1. 5 Protoplast fusion. - 1. 6 Transposition. - 1. 7 Generalized recombination. - 1. 8 Chromosome mapping. - 1. 9 Concluding remarks. - References. - 2 Gene cloning and expression systems in Lactococci. - 2. 1 Introduction. - 2. 2 Replicative gene cloning. - 2. 3 Integrative gene cloning. - 2. 4 Gene expression signals. - 2. 5 Control of gene expression. - 2. 6 Protein secretion. - 2. 7 Expression and secretion systems. - 2. 8 Food grade systems. - References. - 3 Bacteriophages and bacteriophage resistance. - 3. 1 Introduction. - 3. 2 Types and species of bacteriophages. - 3. 3 Gene directed bacteriophage resistance in lactic acid bacteria. - 3. 4 Novel phage defense mechanisms. - 3. 5 Genetic strategies to construct phage-insensitive strains. - 3. 6 Conclusions and perspectives. - References. - 4 The proteolytic system of lactic acid bacteria. - 4. 1 Introduction. - 4. 2 Proteinases. - 4. 3 Endopeptidases. - 4. 4 General aminopeptidases. - 4. 5 Proline-specific peptidases. - 4. 6 Oligo-and carboxypeptidases. - 4. 6. 1Dipeptidases. - 4. 7 Transport of amino acid and peptides. - 4. 8 Cellular localization of proteolytic enzymes. - 4. 9 Engineering of the proteolytic system. - References. - 5 Bacteriocins of lactic acid bacteria. - 5. 1 Introduction. - 5. 2 Small heat-stable bacteriocins. - 5. 3 Large heat-labile bacteriocins. - 5. 4 Lantibiotics in lactic acid bacteria. - 5. 5 Concluding remarks. - References. - 6 Genetic engineering of lactobacilli, leuconostocs and Streptococcus thermophilus. - 6. 1 Introduction. - 6. 2 Overview of taxonomy and health benefits. - 6. 3 Biochemical traits. - 6. 4 Plasmid biology and cloning vectors. - 6. 5 Genetic transfer methods. - 6. 6 Gene expression in lactobacilli. - 6. 7 Gene expression in S. thermophilus and leuconostocs. - 6. 8 Chromosomal integration of genes. - 6. 9 Phage and phage resistance. - 6. 10 Concluding remarks. - Dedication and acknowledgements. - References.
