Tick-Borne Viruses

This volume covers a variety of the latest scientific methods utilized in tick-borne virus (TBV) research. The chapters in this book explore detailed protocols on how to conduct in-depth research on TBVs; enhance understanding of the virus biology; transmission mechanisms; and the creation of effective countermeasures. Following the established Methods in Molecular Biology series format, each chapter introduces the topic, lists necessary materials and reagents, offers step-by-step reproducible laboratory protocols, and includes troubleshooting tips to help avoid common issues.

Cutting-edge and thorough, Tick-Borne Viruses: Methods and Protocols is a comprehensive and innovative resource for researchers, clinicians, and public health experts interested in exploring the complexities of TBVs.

Tick Cell Culture Methods. - Virus Isolation and Culture from Field Collected Ticks: In Vitro. - Virus Isolation from Field-Collected Ticks: In Vivo. - Generation of Ticks Infected with Tick-Borne Encephalitis Virus. - Generation of Virus Infected Ticks: Synchronous Infection and Feeding on Viremic Animals. - Human Skin Ex-Vivo Culture for Tick-Borne Virus Infection Studies. - Colorado Tick Fever Virus: Quantification, Propagation, Storage, and Detection. - Isolation and Quantitation of Dabie bandavirus. - Immunofluorescence Assays to Visualize Tick-Borne Flaviviruses in Mouse Skin or Brain. - Immunohistochemical Assays to Visualize Tick-Borne Flaviviruses at the Tick Feeding Site. - RNA In-Situ Hybridization to Visualize Tick-Borne Viruses. - Quantification of Tick-Borne Flaviviruses Using Focus Forming Assays. - Quantification of Neutralizing Antibodies to Tick-Borne Viruses using Focus Neutralization Assays. - Methods for Evaluation of Antivirals and Neutralizing Antibodies Against Tick-Borne Encephalitis Virus. - Exploring Tick-Borne Encephalitis Virus-Host Cell Interactions using Electron Tomography: Methodologies and Protocols.