Cyanobacteria Biotechnology

Guides the reader through the interesting and useful aspects of cyanobacteria metabolism but will keep true to a biotechnology vision.

1 Inorganic carbon assimilation in cyanobacteria: mechanisms, regulation and engineering
1. 1 Introduction - the need for a carbon-concentrating mechanism
1. 2 The carbon-concentrating mechanism (CCM) among cyanobacteria
1. 3 Regulation of Ci assimilation
1. 4 Engineering the cyanobacterial CCM
1. 5 Photorespiration
1. 6 Concluding remarks

2 Electron transport inc yanobacteria and its potential in bioproduction
2. 1. Introduction
2. 2. Electron transport in a bioenergetic membrane
2. 3. Respiratory electron transport
2. 4 The role of electron sinks in photoprotection
2. 5 Regulating electron flux into different pathways
2. 6 Spatial organisation of electron transport complexes
2. 7 Manipulating electron transport for synthetic biology applications
2. 8 Future challenges in cyanobacterial electron transport

3 Optimizing the spectral fit between cyanobacteria and solar radiation in the light of sustainability applications
3. 1 Introduction
3. 2 The molecular basis and efficiency of Oxygenic Photosynthesis
3. 3 Fit between the spectrum of solar radiation and the action spectrum of photosynthesis
3. 4 Expansion of the PAR region of Oxygenic Photosynthesis
3. 5 Modulation and optimization of the transparency of photobioreactors
3. 6 Full control of the light regime: LEDs inside the PBR
3. 7 Conclusions and prospects

4 What we can learn from measuring metabolic fluxes in cyanobacteria
4. 1 Central carbon metabolism in cyanobacteria: an overview and renewed pathway knowledge
4. 2 Methodologies for predicting and quantifying metabolic fluxes in cyanobacteria
4. 3 Cyanobacteria fluxome in response to altered nutrient modes and environmental conditions
4. 4 Metabolic fluxes redirected in cyanobacteria for biomanufacturing purposes
4. 5 Synopsis and future directions

5 Synthetic biology in cyanobacteria and applications for biotechnology
5. 1 Getting genes into cyanobacteria
5. 2 Basic synthetic control of gene expression in cyanobacteria
5. 3 Exotic signals for controlling expression
5. 4 Advanced regulation: the near future
5. 5 Conclusions

6 Sink Engineering in Photosynthetic Microbes
6. 1 Introduction
6. 2 Source and Sink
6. 3 Regulation of Sink Energy in Plants
6. 4 Engineered pathways as sinks in Photosynthetic Microbes
6. 5 What are key source/sink regulatory hubs in photosynthetic microbes?
6. 6 Concluding Remarks

7 Design principles for engineering metabolic pathways in cyanobacteria
7. 1 Introduction
7. 2 Cofactor optimization
7. 3 Incorporation of thermodynamic driving force into metabolic pathway design
7. 4 Development of synthetic pathways for carbon conserving photorespiration and enhanced carbon fixation
7. 5 Summary and future perspective on cyanobacterial metabolic engineering

8 Engineering cyanobacteria for efficient photosynthetic production: ethanol case study
8. 1 Introduction
8. 2 Pathway for ethanol synthesis in cyanobacteria
8. 3 Selection of optimal cyanobacteria ? chassis, ? strain for ethanol production
8. 4 Metabolic engineering strategies toward more efficient and stable ethanol production
8. 5 Exploring the response in cyanobacteria to ethanol
8. 6 Metabolic engineering strategies to facilitate robust cultivation against biocontaminants
8. 7 Conclusions and Perspectives

9 Engineering cyanobacteria as host organisms for production of terpenes and terpenoids
9. 1 Terpenoids and industrial applications
9. 2 Terpenoid biosynthesis in cyanobacteria
9. 3 Natural occurrence and physiological roles of terpenes and terpenoids in cyanobacteria
9. 4 Engineering cyanobacteria for terpenoid production
9. 5 Summary and outlook

10 Cyanobacterial biopolymers
10. 1 Polyhydroxybutryate
10. 2. Cyanophycin granules in cyanobacteria

11 Biosynthesis of fatty acid derivatives by cyanobacteria: from basics to biofuel production
11. 1 Introduction
11. 2 Overview of fatty acid metabolism
11. 3 Basic technologies for production of free fatty acids
11. 4 Advanced technologies for enhancement of free fatty acid production
11. 5 Hydrocarbon production in cyanobacteria
11. 6 Advanced technologies for enhancement of hydrocarbon production
11. 7 Basic technologies for production of fatty alcohols
11. 8 Advanced technologies for enhancement of fatty alcohol production
11. 9 Basic technologies for production of fatty acid alkyl esters
11. 10 Perspectives

12 Product export in cyanobacteria
12. 1 Introduction
12. 2 Secretion mediated by membrane-embedded systems
12. 3. Membrane vesicle-mediated secretion
12. 4 Concluding remarks

13 Harnessing Solar-Powered Oxic N2-fixing Cyanobacteria for the BioNitrogen Economy
13. 1 Introduction
13. 2 Physiology and Implications of Oxic Nitrogen Fixation
13. 3 Major Biotechnology Applications for Diazotrophic Cyanobacteria
13. 4 Conclusions

14 Traits of fast-growing cyanobacteria
14. 1 Introduction
14. 2 Why is growth rate significant?
14. 3 An overview of factors affecting growth rates of cyanobacteria
14. 4 An overview of the fast-growing model cyanobacteria
14. 5 Relationship between light usage and growth rate in model strains
14. 6 Molecular determinants of fast growth of S. elongatus UTEX 2973
14. 7 Carbon fluxes in fast-growing strains determined using metabolic flux analysis (MFA)
14. 8 Engineering cyanobacteria for fast growth
14. 9 Conclusion

15 Cyanobacterial biofilms in natural and synthetic environments
15. 1 Motivation
15. 2 Introduction to biofilms: biology and applications
15. 3 Cyanobacteria in natural biofilms and microbial mats
15. 4 Introduction to (Photo-)biotechnology
15. 5 Benefits of microscale systems for (photo-)biofilm cultivation
15. 6 Oxygen accumulation and its impacts
15. 7 Resource management in biofilms
15. 8 Applications of photosynthetic biofilms
15. 9 Outlook

16 Growth of Photosynthetic Microorganisms in Different Photobioreactors Operated Outdoors
16. 1 Background
16. 2 Case studies of Outdoor Cultivations of Photosynthetic Microorganisms
6. 3 Conclusion