Knowledge of the structure of biological macromolecules forms the basis of understanding their function and their mechanism of action, and lays the foundation for rational protein and drug design. The only method to obtain this knowledge is crystallography. This book provides guidelines, and is aimed at biochemists and protein crystallographers.
Inhaltsverzeichnis
1. Useful Principles for the Crystallization of proteins 2. Detergent Phenomena in Membrane Protein crystallization 3. General and Practical aspects of membrane protein crystallization 4. Crystallizing Membrane Proteins 5. Protein-Detergent Micellar Solutions for the Crystallization of Membrane Proteins 6. Crystallization of purple Bacterial Antenna Complexes 7. Crystallization of Reaction Centers from Rhodobacter sphaeroides 8. Crystallization of the Light-Harvesting Chlorophyll a/b Protein Complex from Chloroplast Membranes 9. The crystallization of the Ca2 -ATPase of Sarcoplasmic Reticulum 10. Two-Dimensional Crystals of the Rhodoseudomonas viridis Photosynthetic Reaction Center 11. Preparation of Membrane Crystals of Mitochondrial
