Inhaltsverzeichnis
Electrophoresis of RNA Denatured with Glyoxal or Formaldehyde. - Northern Blotting. - Hybrid-Release Translation. - Hybrid-Arrested Translation. - In Vitro Translation and Analysis of Early Events in Protein Synthesis Initiation in Nonreticulocyte Mammalian Cells. - Use of Elutips to Purify DNA. - Plasmid Preparation on Sephacryl S1000. - Electrophoresis of DNA in Nondenaturing Polyacrylamide Gels. - Use of 35S Nucleotides for DNA Sequencing. - Direct Dideoxy DNA Sequencing. - Computer Applications to Molecular Biology. - Detection of Sequence-Specific Protein-DNA Interactions by the DNA-Footprinting Technique. - Oligonucleotide Synthesis Using the Manual Phosphotriester Method. - Manual Oligonucleotide Synthesis Using the Phosphoramidite Method. - Purification of Synthetic Oligonucleotides by Preparative Gel Electrophoresis. - Isolation of High Molecular Weight DNA Suitable for the Construction of Genomic Libraries. - Construction of Mammalian Genomic Libraries Using ? Replacement Vectors. - Cosmid Library Construction. - Construction of cDNA Libraries in ? gt10 or ? gt11. - Immunoscreening of ? gt11 Expression Libraries Using an Avidin Biotin Detection System. - Primed Synthesis and Direct Sequencing in the Isolation of cDNA Clones Using Short Oligonucleotides. - Constructing Expression cDNA Libraries Using Unphosphorylated Adaptors. - Expression Screening of cDNA Libraries in pEX. - Producing Antibodies of Predetermined Specificity from Escherichia coli Hybrid Proteins. - Epitope Mapping Using pEX. - DNA Transfection of Mammalian Cells Using Polybrene. - Expression of Foreign Genes in Mammalian Cells. - Dot-Blot Hybridization Method. - Detection of Unique or Low Copy Number DNA Sequences in Complex Genomes. - Photobiotin-Labeled DNA and RNA Hybridization Probes. - Enzyme-Labeled DNA Probes. -Luminescent Detection of Specific DNA Sequences. - Detection of DNA Sequences Using Biotinylated Probes. - Crosslinking of Single-Stranded DNA to Resins. - Extraction of RNA from Plants. - Isolation of Plant Nuclei. - In Vitro Transcription in Plant Nuclei. - Large-Scale Isolation of Ti Plasmid DNA. - Transformation and Regeneration of Dicotyledonous Plants. - Plant Protoplast Fusion. - Plant Tissue Culture. - Direct Gene Transfer into Plant Protoplasts. - Isolation of Wheat Chloroplasts and Chloroplast DNA. - DNA Labeling by Isolated Wheat Chloroplasts.
