Protein Arrays

Protein Arrays: Methods and Protocols is an introduction to protein array technology and its application to the multiplexed detection of proteins. Although protein array technology has some roots in gene array technology, it can only be described as a distant relative. Unlike DNA, with its established rules of base pairing, and therefore predictable biochemical behavior, proteins are rich with diversity. Proteins can be large or small, compact or extended, basic or acidic, hydrophobic or hydrophilic, and so on. Just as importantly, their behavior is determined by the environment in which they reside, and so the composition of the buffer in which experiments are performed has a dramatic impact on the outcome of the experiment. Thus, if the goal is to simultaneously measure the expression of a large number of proteins, these variables must be addressed. Not to be deterred, scientists have created a variety of solutions to successfully detect and ch- acterize multiple proteins simultaneously. It is the intent of this volume to introduce to the reader a set of technological solutions to the diversity problem as well as to provide the reader with some examples of practical applications of these technologies.

Protein Arrays From cDNA Expression Libraries. - Protein Expression Arrays for Proteomics. - Generation of Protein In Situ Arrays by DiscernArray Technology. - Multiplexed Protein Analysis Using Spotted Antibody Microarrays. - Target-Assisted Iterative Screening of Phage Surface Display cDNA Libraries. - Design, Construction, and Use of Tissue Microarrays. - A Streptavidin-Biotin-Based Microarray Platform for Immunoassays. - Site-Specific Immobilization of Biotinylated Proteins for Protein Microarray Analysis. - Processing of Photoaptamer Microarrays. - Use of a Small Molecule-Based Affinity System for the Preparation of Protein Microarrays. - Evaluation of Antibodies and Microarray Coatings As a Prerequisite for the Generation of Optimized Antibody Microarrays. - Printing Functional Protein Microarrays Using Piezoelectric Capillaries. - The Use of Precision Glass Syringes and a Noncontact Microsolenoid Dispenser for the Production of High-Throughput Low-Density Arrays. - A Protein Microarray ELISA for Screening Biological Fluids. - Protein-Domain Microarrays. - SH3 Domain Protein-Binding Arrays. - Site-Specific Peptide Immobilization Strategies for the Rapid Detection of Kinase Activity on Microarrays. - Simultaneous Monitoring of Multiple Kinase Activities by SELDI-TOF Mass Spectrometry. - Cytokine Protein Arrays. - Fabrication and Application of G Protein-Coupled Receptor Microarrays. - ProteinChip® Array-Based Amyloid ? Assays. - Serum Protein-Expression Profiling Using the ProteinChip® Biomarker System. - Protein Arrays for Serodiagnosis of Disease.