Proteomics in Biology, Part A

Proteomics in Biology Part A, the latest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field, and a focus on proteomics for this updated volume.

1. An Easy and Fast Protocol for Affinity Bead-Based Protein Enrichment and Storage of Proteome Samples
A. Otto, S. Maaß, F. Bonn, K. Büttner and D. Becher
2. Filter Aided Sample Preparation the Versatile and Efficient Method for Proteomic Analysis
J. R. Wisniewski
3. An Overview of Advanced SILAC Labeling Strategies for Quantitative Proteomics
F. Terzi and S. Cambridge
4. Label Free and Standard Free Absolute Quantitative Proteomics Using the 'Total Protein' and 'Proteomic Ruler' Approaches
J. R. Wisniewski
5. Separation of Two Distinct O-Glycoforms of Human IgA1 by Serial Lectin-Chromatography Followed by Mass Spectrometry O-Glycan Analysis
S. Lehoux and T. Ju
6. Monitoring Protein Synthesis in Caenorhabditis Elegans Using SILAC
M. Miyagi
7. Identification of Novel Macropinocytosing Human Antibodies by Phage Display and High-Content Analysis
K. D. Ha, S. M. Bidlingmaier, Y. Su, N. -K. Lee and B. Liu
8. Beyond the Natural Proteome: Non-Degenerate Saturation Mutagenesis - Methodologies and Advantages
M. M. Ferreira Amaral, L. Frigotto and A. V. Hine
9. WGCNA Application to Proteomic and Metabolomic Data Analysis
G. Pei, L. Chen and W. Zhang
10. A Protocol for Large-Scale Proteomic Analysis of Microdissected Formalin Fixed and Paraffin Embedded Tissue
P. Ostasiewicz and J. R. Wisniewski
11. Body Fluid Degradomics and Characterization of Basic N-Terminome
F. Sabino, O. Hermes and U. auf dem Keller
12. Reannotation of Genomes by Means of Proteomics Data
J. Armengaud
13. Proteogenomics: Recycling Public Data to Improve Genome Annotations
A. McAfee and L. J. Foster
14. Targeted in-Depth Quantification of Signaling Using Label-Free Mass Spectrometry
P. R. Cutillas
15. Biotin Switch Assays for Quantitation of Reversible Cysteine Oxidation
R. Li and J. Kast
16. Application of a Thioredoxin Trapping Mutant for Analysis of the Cellular Nitrosoproteome
M. Benhar
17. Combining Click-Chemistry Based Proteomics with Dox-Inducible Gene Expression
J. Gebert, M. Schnölzer, U. Warnken and J. Kopitz
18. Analysis of the Proteome of Hair-Cell Stereocilia by Mass Spectrometry
J. F. Krey, P. A. Wilmarth, L. L. David and P. G. Barr-Gillespie
19. Proteomic Analysis of Metabolic Responses to Biofuels and Chemicals in Photosynthetic Cyanobacteria
T. Sun, L. Chen and W. Zhang
20. Deep Profiling of Proteome and Phosphoproteome by Isobaric Labeling, Extensive Liquid Chromatography and Mass Spectrometry
B. Bai, H. Tan, V. R. Pagala, A. A. High, V. P. Ichhaporia, L. Hendershot and J. Peng
21. Glycoprotein Enrichment Analytical Techniques: Advantages and Disadvantages
R. Zhu, L. Zacharias, K. M. Wooding, W. Peng and Y. Mechref
22. Quantitative Glycomics: A Combined Analytical and Bioinformatics Approach
L. Veillon, S. Zhou and Y. Mechref