The Special Issue on the "Rational Use of Feed to Promote Animal Healthy Feeding" explores the critical intersection of animal nutrition, health, and sustainability. Currently, facing real and demanding challenges (climate change, resource scarcity, and an increasing population) in ensuring global food production while adopting an integrated animal husbandry strategy has become essential and mandatory. The utilization and valorization of underutilized feed resources or by-products from the agri-food sector for animal farming can represent an agricultural practice that provides a sustainable feeding solution within the context of a circular bioeconomy. However, incorporating different technologies or alternative ingredients that are currently available, such as fermentation processes, probiotic supplements, natural additives dietary inclusion, agri-food by-products, and alternative protein sources, can be used to offer comprehensive insights into the complex issue of rational feed utilization while maintaining productive performances, enhancing nutritional animal products and their intestinal health.