Directed Evolution Library Creation

Error-Prone PCR and Effective Generation of Gene Variant Libraries for Directed Evolution. - Error-Prone Rolling Circle Amplification Greatly Simplifies Random Mutagenesis. - Random Mutagenesis by Error-Prone Pol Plasmid Replication in Escherichia coli. - The Sequence Saturation Mutagenesis (SeSaM) Method. - Generation of Effective Libraries by Neutral Drift. - Site Saturation Mutagenesis. - Iterative Saturation Mutagenesis (ISM): A Powerful Approach to Engineer Proteins by Systematically Simulating Darwinian Evolution. - Generating Targeted Libraries by the Combinatorial Incorporation of Synthetic Oligonucleotides During Gene Shuffling (ISOR). - OmniChange: Simultaneous Site-Saturation of up to Five Codons. - Random Insertional Deletional Strand Exchange Mutagenesis (RAISE): A Simple Method for Generating Random Insertion and Deletion Mutations. - Transposon-Based Approaches for Generating Novel Molecular Diversity during Directed Evolution. - Restriction Enzyme-Mediated DNA Family Shuffling. - Assembly of Designed Oligonucleotides (ADO): A Useful Tool in Synthetic Biology for Creating High Quality Combinatorial DNA Libraries. - One-Pot, Simple Methodology for Cassette Randomization and Recombination for Focused Directed Evolution (OSCARR). - USER Friendly DNA Recombination (USERec): Gene Library Construction Requiring Minimal Sequence Homology. - ITCHY: Incremental Truncation for the Creation of Hybrid Enzymes. - Generating Random Circular Permutation Libraries. - Probabilistic Methods in Directed Evolution: Library Size, Mutation Rate and Diversity. - The Mutagenesis Assistant Program. - Computational Tools for Designing Smart Libraries. - Computational Tools for Directed Evolution: A Comparison of Prospective and Retrospective Strategies. - Designing Libraries of Chimeric Proteins Using SCHEMA Recombination and RASPP. - Non-Contiguous SCHEMA Protein Recombination. - Engineering Proteins by Reconstructing Evolutionary Adaptive Paths.