Kinase Screening and Profiling

HTRF Kinase Assay Development and Methods in Inhibitor Characterization. - Application of Eukaryotic Elongation Factor-2 Kinase (eEF-2K) for Cancer Therapy: Expression, Purification, and High-Throughput Inhibitor Screening. - Recombinant Kinase Production and Fragment Screening by NMR Spectroscopy. - Bioluminescence Methods for Assaying Kinases in Quantitative High-Throughput Screening (qHTS) Format Applied to Yes1 Tyrosine Kinase, Glucokinase and PI5P4K Lipid Kinase. - Using Bioluminescent Kinase Profiling Strips to Identify Kinase Inhibitor Selectivity and Promiscuity. - Measuring Activity of Phosphoinositide Lipid Kinases Using a Bioluminescent ADP-Detecting Assay. - A High-Throughput Radiometric Kinase Assay. - A High Content Assay to Screen for Modulators of EGFR Function. - Monitoring Protein Kinase Expression and Phosphorylation in Cell Lysates with Antibody Microarrays. - From Enzyme to Whole Blood: Sequential Screening Procedure for Identification and Evaluation of p38 MAPK Inhibitors. - Genetically Encoded Fluorescent Indicators to Visualize Protein Phosphorylation in Living Cells. - Characterization of an Engineered Src Kinase to Study Src Signaling and Biology. - Screening One-Bead-One-Compound Peptide Libraries for Optimal Kinase Substrates. - Determination of the Substrate Specificity of Protein Kinases with Peptide Micro- and Macroarrays. - Rapid Identification of Protein Kinase Phosphorylation Site Motifs Using Combinatorial Peptide Libraries.