Synthetic Biology

Re-Factoring Glycolytic Genes for Targeted Engineering of Catabolism in Gram-Negative Bacteria. - Negative Regulation Gene Circuits for Efflux Pump Control. - Methods for Generating Microbial Co-Cultures that Grow in the Absence of Fixed Carbon or Nitrogen. - Synthetic Biology with an All
E. coli
TXTL System: Quantitative Characterization of Regulatory Elements and Gene Circuits. - Transformation of an Exotic Yeast Species into a Platform Organism: A Case Study for Engineering Glycolipid Production in the Yeast
Starmerella bombicola. -
Sortase A-Assisted Metabolic Enzyme Ligation in
Escherichia coli
for Enhancing Metabolic Flux. - Construction of CRISPR Libraries for Functional Screening. - Targeted Genome Editing Using DNA-Free RNA-Guided Cas9 Ribonucleoprotein for CHO Cell Engineering. - Dynamic Management of Codon Compression for Saturation Mutagenesis. - Designed Transcriptional Regulation in Mammalian Cells Based on TALE- and CRISPR/dCas9. - Combinatorial Evolution of DNA with RECODE. - Genome Editing in
Penicillium chrysogenum
Using Cas9 Ribonucleoprotein Particles. - Targeted Gene Knockout Using Nuclease-Assisted Vector Integration: Hemi- and Homozygous Deletion of
JAG1. -
Programming Biomaterial Interactions Using Engineered Living Cells. - Rapid Control of Genome Editing in Human Cells by Chemical-Inducible CRISPR-Cas Systems. - Production of a Human Cell Line with a Plant Chromosome. - Bacterial Genome Editing with CRISPR-Cas9: Taking
Clostridium beijerinckii
as an Example. - CRISPR-Cas9-Mediated Genome Editing and Transcriptional Control in
Yarrowia lipolytica. -
Construction and Integration of a Synthetic MicroRNA Cluster for Multiplex RNA Interference in Mammalian Cells. - Bio-Algorithmic Workflows for Standardized Synthetic Biology Constructs. - Engineering Genomes with Genotype Specification Language. - Automated Visualization of Genetic Designs Using DNAplotlib. - DNA-Based Analog Computing. - DNA Assemblywith the DATEL Method. - EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. - Designing and Assembling Plasmids for Construction of
Escherichia coli
Biosensor for
Vibrio cholerae
Detection. - A Rapid Combinatorial Approach to Assembling Synthetic Prokaryotic and Eukaryotic Protein Expression Vectors. - Standardized Cloning and Curing of Plasmids. - STAR: A Simple TAL Effector Assembly Reaction Using Isothermal Assembly.