Biotechnological processes often require the use of immobilized biocatalysts. This translation of the most successful German edition introduces the theoretical background of this rapidly growing field followed by a detailed description of the various techniques of immobilizing, characterizing, and using biocatalysts. The comprehensive discussion of possible applications in industrial processes and in basic research results in a practical guide for everyone involved in these techniques. Furthermore, some selected experiments are added in order to facilitate the understanding of the theoretical reports.
Inhaltsverzeichnis
Theoretical Section.- 1 General Principles.- 2 Methods of Immobilization.- 3 Characteristics of Immobilized Biocatalysts.- 4 Reactors for Immobilized Biocatalysts.- 5 Industrial Applications.- 6 Application in Analytical Procedures.- 7 Uses in Medicine.- 8 Uses in Basic Research.- 9 Special Developments and Trends.- Practical Section.- Exercise 1 Adsorptive Coupling of Invertase to Active Charcoal.- Exercise 2 Ionic Binding of Catalase to CM-Cellulose.- Exercise 3 Covalent Binding of Glucoamylase to a Carrier Bearing Oxirane Groups.- Exercise 4 Immobilization of ß-Galactosidase by Cross-linking.- Exercise 5 Alginate Entrapment of Yeast Cells and Their Co-entrapment with Immobilized ß-Galactosidase.- Exercise 6 Construction and Use of a Biochemical Electrode for Essaying Glucose.- Exercise 7 Spinning of ß-Galactosidase into Cellulose Acetate Fibers.- Exercise 8 Immobilization of L-Asparaginase in Nylon Microcapsules.- Exercise 9 Degradation of Cellulose in a Membrane Reactor.- Exercise 10 Conversion of Fumaric Acid to Malic Acid in a Liquid-membrane Emulsion.- Abbreviations and Symbols.- Literature.