Immobilized Biocatalysts

Biotechnological processes often require the use of immobilized biocatalysts. This translation of the most successful German edition introduces the theoretical background of this rapidly growing field followed by a detailed description of the various techniques of immobilizing, characterizing, and using biocatalysts. The comprehensive discussion of possible applications in industrial processes and in basic research results in a practical guide for everyone involved in these techniques. Furthermore, some selected experiments are added in order to facilitate the understanding of the theoretical reports.

Theoretical Section. - 1 General Principles. - 2 Methods of Immobilization. - 3 Characteristics of Immobilized Biocatalysts. - 4 Reactors for Immobilized Biocatalysts. - 5 Industrial Applications. - 6 Application in Analytical Procedures. - 7 Uses in Medicine. - 8 Uses in Basic Research. - 9 Special Developments and Trends. - Practical Section. - Exercise 1 Adsorptive Coupling of Invertase to Active Charcoal. - Exercise 2 Ionic Binding of Catalase to CM-Cellulose. - Exercise 3 Covalent Binding of Glucoamylase to a Carrier Bearing Oxirane Groups. - Exercise 4 Immobilization of ß-Galactosidase by Cross-linking. - Exercise 5 Alginate Entrapment of Yeast Cells and Their Co-entrapment with Immobilized ß-Galactosidase. - Exercise 6 Construction and Use of a Biochemical Electrode for Essaying Glucose. - Exercise 7 Spinning of ß-Galactosidase into Cellulose Acetate Fibers. - Exercise 8 Immobilization of L-Asparaginase in Nylon Microcapsules. - Exercise 9 Degradation of Cellulose in a Membrane Reactor. - Exercise 10 Conversion of Fumaric Acid to Malic Acid in a Liquid-membrane Emulsion. - Abbreviations and Symbols. - Literature.