EMC 2008

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14th European Microscopy Congress 1-5 September 2008, Aachen, Germany
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Titel: EMC 2008

ISBN: 9783540852285
EAN: 9783540852285
Format:  PDF
Sprache: Englisch.
Dateigröße in MByte: 33.
Springer-Verlag GmbH

31. August 2008 - pdf eBook

Beschreibung

Proceedings of the14th European Microscopy Congress, held in Aachen, Germany, 1-5 September 2008. Jointly organised by the European Microscopy Society (EMS), the German Society for Electron Microscopy (DGE) and the local microscopists from RWTH Aachen University and the Research Centre Jülich, the congress brings together scientists from Europe and from all over the world. The scientific programme covers all recent developments in the three major areas of instrumentation and methods, materials science and life science.

Inhaltsverzeichnis

1;Preface Volume 3: Life Science;5 2;Content;7 3;Cryoelectron microscopy: from molecules to systems;23 4;Progress in High-resolution Scanning Probe Microscopy;25 5;Modular organization of RNase P;27 6;Molecular anatomy of the human pathogen;29 7;Unveiling conformational changes of biological molecules using multiscale modeling and multiresolution experiments;31 8;Nicolas Boisset: In memoriam;33 9;Cryo TEM-based 3D reconstruction of the recombinant expressed human zinc peptidase Meprin ß;35 10;Origin melting by SV40 Large T antigen-new insights from 3D- EM- MLF3D classification method;37 11;Sequence analysis and modelling of the two large subunits of Phosphorylase Kinase;39 12;Cellular Uptake of Polymer Nanoparticles Imaged by Electron Microscopy;41 13;9 Å cryo-EM structure and molecular model of a gastropod hemocyanin didecamer ( KLH1) reveals the architecture of the asymmetric collar;43 14;Microstructure of model systems for sauces based in polysacharides observed by Cryo- SEM;45 15;Three-dimensional architecture of outer- and inner-dynein arms in flagella revealed by cryo- electron tomography and single particle analysis;47 16;Structural and functional studies of rabbit skeletal muscle Phosphorylase Kinase;49 17;Single-particle analysis of the Cdc6/Orc1, archaeal DNA replication initiator protein;51 18;A high-throughput acquisition toolbox in Matlab for cryo- electron microscopy studies;53 19;Architecture of bacterial glutamate synthase complexe using hybrid approaches;55 20;3D Structure of DNA Repair Macromolecular Complexes Participating in Non- Homologous End- Joining ( NHEJ);57 21;Quaternary structure of recombinant human meprin a zinc peptidase of the astacin family;59 22;Allosterism of Nautilus pompilius hemocyanin as deduced from 8 Å cryo-EM structures obtained under oxy and deoxy conditions;61 23;High resolution structure of a 6 MDa protease by xray- crystallography and cryo- EM;63 24;Cryo-TEM of liquid crystalline particles: Application of materials scien
ce techniques to study liquid crystalline particle structures.;65 25;Structural basis for the concerted integration of HIV- 1 DNA in the human genome, role of the human cofactor LEDGF/ p75.;67 26;Titan Krios: Automated 3D imaging at ambient and cryogenic conditions;69 27;Self assembly and phase behaviour of new sugar based gemini amphiphiles;71 28;Peptide-nanotube formation by lysine based lipoamino acids;73 29;Advances in biological microanalysis using event streamed spectrum imaging and programmed beam acquisition;75 30;3D-correlation-averaging for membrane-protein-crystals;77 31;8 Å cryo-EM structure of the giant hemoglobin from the planorbid snail Biomphalaria glabrata;79 32;Structure of Pex5p and Pex5-20 complexes in the yeast Hansenula polymorpha. Pex20p causes a conformational change upon binding to Pex5p tetramers involved in peroxisomal protein transport.;81 33;Structure of OprM-MexA interacting complex revealed by cryo electron tomography;83 34;Pleiomorphic viruses revealed by cryo tomography: the structure of coronaviruses;85 35;Combined structural and chemical analysis of unique anammox bacteria that contain a prokaryotic organelle;87 36;Structural and functional considerations on the 3- D organization of the fenestrated cytoplasm of hepatic endothelial cells;89 37;Molecular architecture of the presynaptic compartment studied by cryo- electron tomography;91 38;Synapses in high pressure frozen Caenorhabditis elegans;93 39;High resolution EM-tomography of a whole vitrified cell;95 40;Evaluation of beam damage in catalase crystals observed in vitrified sections;97 41;Urothelial fusiform vesicles are formed in post- Golgi compartment;99 42;Localisation of GFP and GFP tagged PKD2 in cultivated pancreatic cancer cells using high- pressure freezing;101 43;Cytomegalovirus membrane envelopment revisited a STEM- tomography study based on high- pressure freezing and freeze substitution.;103 44;Electron tomography & template matching of biological membranes;105 45;C
orrelated Microscopy: From Dynamics to Structure;107 46;3DEM-analyses of Golgi apparatus subunits during physiologic and pathologic reorganizations;109 47;Visualization of the 80s ribosome in situ using cryo electron tomography of vitreous sections;111 48;Three-dimensional analysis of the intermediate filament network using SEM- tomography;113 49;The apical vesicles of the tuft cells in the main excretory duct of the rat submandibular gland by EFTEM- TEM tomography;115 50;(S)TEM Dual Axis Tomography;117 51;STEM tomography of high-pressure frozen cell monolayers;119 52;Multimodality of pericellular proteolysis in cancer cell invasion;121 53;Photonic crystal type nanoarchitectures in butterfly wing scales;123 54;Iridovirus-like viruses of Lacerta monticola from Serra da Estrela, Portugal;125 55;Structural and ultrastructural investigations on the in vitro effects of plant extracts in chicken and rabbit whole blood cultures;127 56;Fine structural characterization of cloned human endogenous retrovirus HERV- K113;129 57;The procaine hydrochlorate effect onto the corpuscular anthocyans from the vacuolar sap of different plant cells;131 58;Comparative anatomical and ultrastructural investigations on normal and vitroplantlets leaves from Pistia stratiotes L.;133 59;Autophagy of mitochondria in brown adipocytes of chemically thyroidectomised rats;135 60;Ultrastructural studies of immune organs in antigen primed chickens treated with vegetal extractions;137 61;Structural features of HIV-1 immunologic activation;139 62;Functions of actin-binding proteins in the cell nucleus;141 63;Ultrastructural mechanisms of entamoebae movement system;143 64;White adipocytes transdifferentiation into brown adipocytes induced by triiodothyronine;145 65;Ultrastructural studies of the tegument of cestodes ( Platyhelminthes): phylogenetic implications;147 66;Crystallization stages of the CaCO3 deposits in the earthworms calciferous gland;149 67;Scanning electron microscopic analysis of heavy met
al resistant microorganisms;151 68;Characterization of mouse embryoid bodies by Scanning Electron Microscopy ( SEM) and Confocal Laser Scanning Microscopy ( CLSM);153 69;Stage-dependent localization of mitochondrial DNA during the cell cycle of Euglena gracilis Z by immunogold electron microscopy;155 70;The virus-inducted structures in RNA virus infected macrophages;157 71;Surface layers of ore-leaching Bacteria and Archaea;159 72;Structure of Bacillus anthracis spores analyzed by CEMOVIS;161 73;Insights into the role of pUL71 during HCMV morphogenesis;163 74;Fine structure of the genital chamber of female Chrysomya megacephala (F.) (Diptera: Calliphoridae);165 75;Ultrastructure of the testes and spermatozoa of the blow fly Chrysomya megacephala (F.) (Diptera:Calliphoridae);167 76;Biosynthesis of cellulose in red algae;169 77;Light and electron microscopy aspects of the glandular sessile hairs from the vitroplantlet leave of Drosera rotundifolia;171 78;Ultrastructure of selected Euglena species in relation to their taxonomy;173 79;Confocal Microscopy Reveals Molecular Interactions Between DNA- binding Drugs and Chromatin in Live Cells;175 80;Fluorescence microscopy methods for measuring the mobility and stability of molecules in 3- D samples;177 81;Phasor-FLIM analysis of FRET for homotypic and heterotypic non- covalent interactions: stimulated membrane receptors in live cells;179 82;In vitro immunofluorescence and ultrastructural analysis of the expression of noncollagenous matrix proteins in human pulpal cells induced by mineralizing factors;181 83;Differentiation-dependent Golgi fragmentation in the bladder urothelial cells in vitro;183 84;Monitoring Mitochordria Dynamics in Living Cell System by Confocal Microscopy;185 85;The freeze-fracture replica immunolabeling technique for the study of lipid droplet biogenesis and function;187 86;Dynamics of epigenome duplication, accessibility and translation;189 87;Controlled light exposure microscopy reveals telomeric mi
croterritories throughout the cell cycle;191 88;Calibration in fluorescence correlation spectroscopy for measurements of stem cell differentiation kinetic;193 89;Spectral methods for cells and particles identifications;195 90;3D realistic visualization of supramolecular assemblies;197 91;Heterosis in Arabidopsis thaliana: Structural aspects in mature and germinating seeds from hybrid and parental lines;199 92;Colour visualization of red blood cells in native smears by the new method reflected light microscopy;201 93;In vitro culture of Trigonella foenum-graecum plantules and their anatomic characterization;203 94;Live cell imaging by SEM-hosted X-ray microscope in water window energy range;205 95;Microscopic anatomy and mineral composition of cuticle in amphibious isopods Ligia italica and Titanethes albus (Crustacea:Isopoda);207 96;In vivo imaging and quantification of the continuous keratin filament network turnover;209 97;Necrotic cell death, a controlled way of cellular explosion;211 98;Dissecting mitochondrial protein distributions using sub- diffraction resolution fluorescence microscopy;213 99;High Thoughput, High Content Tissue Cyotometry;215 100;Focus on the vascular wall: Imaging of large arteries using two- photon microscopy;217 101;High resolution confocal Ca2+ imaging of the pulmonary neuroepithelial body microenvironment in lung slices;219 102;Two photon excitation microscopy and SHG imaging as a tool for visualisation of type I and type II collagen, and their use in tissue engineering;221 103;Testing calibration standards for confocal and two- photon microscopy;223 104;Distribution of the voltage-gated delayed-rectifier K+ subunits, Kv1.1 and Kv1.2, in the adult murine enteric nervous system;225 105;Observation of Ventricular Myocyte Morphology in Long Term Culture using High Resolution Confocal Imaging;227 106;A magnetic field enhances the inmunofluorescence signal in a lymphocytic cell;229 107;Novel 515 nm DPSS-laser brings excitement in life cell i
maging;231 108;Confocal laser scanning microscopy study on human fissure caries stained with alizarin red;233 109;Non-invasive skin tissue characterization using non- linear spectral imaging microscopy;235 110;Force Spectroscopy reveals mechanical structure of capsids;237 111;Manipulating and imaging molecular motors with optical traps, single- molecule fluorescence and atomic force microscopy;239 112;Mechanical stability of ethanol-induced A-DNA;241 113;Manipulation of the mechanical properties of a virus by protein engineering;243 114;A single-molecule mechanical assay to study DNA replication coupled to DNA unwinding;245 115;Generation of multiple DNA lesions at subnuclear resolution by multi- photon irradiation;247 116;Light and electron microscopy of phagosomes;249 117;The Use of Electron Microscopy in the Diagnosis of Human Microsporidial Infection the Manchester, UK, Experience;251 118;Overview of the impact of biological microanalysis in health and disease;253 119;Immunolocalization of FOXP3 in HCV-infected liver biopsies. Preliminary observations.;255 120;Ultrastructural and Intracellular Elemental Composition Analysis of Human Hematopoietic Cells During Cold Storage in Preservation Solutions;257 121;Reconstitution and morphological characterisation of an original Human Endogenous Retrovirus;259 122;Cell death in human articular chondrocyte: an ultrastructural study in micromass model.;261 123;Pathomorphological diagnostic of paraffin embedded versus epon embedded cardiac tissues with transmission electron microscope analysis;263 124;Interaction of carbon nanotubes with macrophages: STEM and EELS approach;265 125;The ultastructural effect of nitrogen mustard gas on rat brain and therapeutic value of proanthocyanidin;267 126;Assembly and maturation of pestiviruses;269 127;Analysis of radiation-induced bystander effects using high content screening;271 128;Electron microscopic investigations on normal and dexamethasone applied rat placentas;273 129;Changes in
dental enamel birefringence after CO2 laser irradiation through fluoride gel a pilot study;275 130;SEM and light microscopic examination and morphometric analysis of temporal changes in intimal and medial connective tissue component of human carotid arteries;277 131;Ultrastructure of human amniotic membrane covering villous chorion and smooth chorion in preeclampsia;279 132;Changes in Intracellular Sodium, Chlorine, and Potassium Content in Hematopoietic Cells after Hypotermic Storage;281 133;Requirements for a bright future of electron microscopy in the rapid laboratory diagnosis of infectious diseases;283 134;Changed thickness of zona pellucida as a result of stimulated ovulation;285 135;Mozaic microscopy of pancreatic islets;287 136;Microscope history Database;289 137;Interaction of H-Ras Transformed Cell Line with Folic Acid Modified Magnetic Nanoparticles and Detection by Transmission Electron Microscope;291 138;Sedimentation of suspensions for diagnostic thin section electron microscopy;293 139;Evaluation of rofecoxib administration influence on ultrastructural image of the kidney;295 140;Microstructural changes produced by Pulsed Electric Fields in liquid whole egg studied by Transmission Electron Microscopy ( TEM);297 141;Microstructural changes in dough treated by Glucose oxidase ( GOX) and Transglutaminase ( TG), studied by Scanning Electron Microscopy ( SEM);299 142;Hyperspectral imaging a novel concept for marker free chromosome characterization;303 143;Poxviruses: Morphogenesis of an Orthopoxvirus (CPXV) and an Avipoxvirus ( FPXV) in host tissue;305 144;Ultrastructural alterations of methotrexate in mouse kidney;307 145;Ultrastructural gadolinium evidence in skin of a patient with Nephrogenic Fibrosing Dermopathy;309 146;Nephrotoxicity induced by inorganic Hg(II) and Pb(II): a microscopic and biochemical in vitro study;311 147;Tissue distribution of peroxisomes in zebrafish;313 148;An analysis of the ultrastructure of matured HIV;315 149;Ultrastructura
l analysis of lysosomal storage diseases: effects of therapy;319 150;Immunolabeling for Electronmicroscopy;321 151;Molecular Organisation of Cadherins in Intercellular Adhesion Junctions by Cryo- Electron Tomography of Vitreous Sections;323 152;Ultrastructural observations of indium in the lactating mammary gland cells;325 153;An active mechanism flanks and modulates the export of the small ribosomal subunits;327 154;New methods for micro-domain detection in bacterial sacculi;329 155;Subcellular localization of Myogenic Regulatory Factors along skeletal muscle development;331 156;Immunocytochemical Strategies: LR Resins or Lowicryl, Gold or Peroxidase Which is Better?;333 157;Elemental Analysis in Electron Microscopy for Medical Diagnostics;335 158;Synaptic localization of KCa1.1 potassium channels in central neurons revealed by postembedding immunogold and SDS- digested freeze- fracture replica labelling;337 159;Ultrastructural studies of rod photoreceptor nuclei from SCA7 mouse;339 160;Application of electron energy loss spectroscopy for heavy metal localization in unicellular algae;341 161;Localization of flagella proteins in the Archaeon Ignicoccus hospitalis;343 162;Detection of gold nanoparticles by Autrata YAG detector in FE SEM working in cryo mode;345 163;Correlative cryo-fluorescence and electron microscopy;347 164;The effect of hypometabolizing molecules on transcription as shown by a new two- step pulse- chase method;349 165;Cryofixation and Freeze-Substitution Combined with Tokuyasu Cryo- section Labelling;351 166;The Drosophila active zone architecture: combining confocal, STED and transmission electron microscopy;353 167;Distribution and function of amorphous CaCO3 and calcite within the tergite cuticle of terrestrial isopods (Crustacea);355 168;A symbiosis: tracking cell signaling with expression probes, quantum dots and a programmable array microscope ( PAM);357 169;Golgi twins in mitosis revealed by genetically encoded tags for live cell imaging an
d correlated electron microscopy;359 170;Understanding the 3D architecture of organelle bound protein complexes using cryo- electron tomography of frozen hydrated sections and immunogold; our next great challenge;361 171;Cryo-electron microscopy of vitreous sections;363 172;The visualization of viruses in the low- voltage electron microscope;365 173;Exploring HPF/FS methods for sensitive immunogold labeling on resin sections;367 174;Low contrast of the ER-membranes in the high pressure frozen, freeze substituted specimens;369 175;HPF of cultured cell monolayers: towards a standard method for high pressure freezing, freeze substitution and electron tomography acquisition;371 176;Life-like physical fixation of large samples for correlative microscopy;373 177;Integrating automated single, double and triple in situ molecular detection with imaging for the analysis of inter- and intracellular events in the seminiferous tubule;375 178;Freeze-substitution in Epon: An attempt to combine immunolabeling and improvement of structural preservation;377 179;The Shape of Caveolae is Omega-like after Chemical Fixation and Cup- like after Cryofixation;379 180;Integrated laser and transmission electron microscopy;381 181;Correlative 3D microscopy: CLSM and FIB/SEM tomography used to study cellular entry of vaccinia virus;383 182;Strategies for the morphological analysis of hydrated and life- like preserved biomedical material in the SEM;385 183;Denaturation of metaphase chromatin plates observed by transmission electron microscopy and atomic force microscopy in aqueous solution;387 184;Discovery of a Nuclear Structure in UVC-induced Apoptotic Cells by Integrated Laser Electron Microscopy;389 185;Analysis of biomineral formation in three-dimensional micro- mass stem cell cultures;391 186;Comparative structural studies of highly-filled polymethacrylates with light microscopy, SEM, TEM and AFM;393 187;Cell surface characteristics as reporters for cellular energy state;395 188;Analysis o
f multimodal 3D microscopy measurements;397 189;Permanent plastid nuclear complexes (PNCs) in plant cells;399 190;Cell imaging by dynamic secondary ion mass spectrometry ( SIMS): basic principles and biological applications;401 191;Water imaging in cells cryosections by electron energy loss spectroscopy;403 192;Study of the cellular uptake of Pt nanoparticles in human colon carcinoma cells;405 193;Ion spectrometry and electron microscopy correlative imaging for accurate comprehensive trace element localization at subcellular level, in normal and pathological keratinocytes;407 194;Calcium Spark Detection and Analysis in Time Series of Two- Dimensional Confocal Images;409 195;Visualisation of the attachment, possible uptake and distribution of technical nanoparticles in cells with electron microscopy methods;411 196;Author Index;413 197;Subject Index;423


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