Stevia rebaudiana Bert (Asteraceae) has garnered attention with the rise in demand for low-carbohydrate, low-sugar food alternatives. It has also shown promise in medical research for treating such conditions as obesity and high blood pressure. Stevia rebaudiana Bert (Asteraceae) has a negligible effect on blood glucose, even enhancing glucose tolerance, therefore, it is attractive as a natural sweetener to diabetics and others on carbohydrate-controlled diets. It has also shown promise in medical research for treating such conditions as obesity and high blood pressure. Stevia has a negligible effect on blood glucose, even enhancing glucose tolerance, therefore, it is attractive as a natural sweetener to diabetics and others on carbohydrate-controlled diets. In the present research we have taken a mother plant of Stevia rebaudiana Bert (Asteraceae) and cultured it in tissue culture lab in order to avoid contamination.