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Wound Healing

Methods and Protocols. 'Methods in Molecular Medicine'. Auflage 2003. Book. Sprache: Englisch.
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During the past decade, significant progress in molecular and cellular te- niques has greatly advanced our understanding of the wound healing p- cess. Many of these new techniques have been utilized in the context of more classic models of wound heal … weiterlesen
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Titel: Wound Healing

ISBN: 0896039994
EAN: 9780896039995
Methods and Protocols.
'Methods in Molecular Medicine'.
Auflage 2003.
Book.
Sprache: Englisch.
Herausgegeben von Aime L. Burns, Luisa A. DiPietro
Humana Press

20. Januar 2003 - gebunden - 484 Seiten

Beschreibung

During the past decade, significant progress in molecular and cellular te- niques has greatly advanced our understanding of the wound healing p- cess. Many of these new techniques have been utilized in the context of more classic models of wound healing. The combination of new and classic approaches has allowed scientists to make exciting discoveries in the field of tissue repair, resulting in an explosion of information about the healing p- cess. Importantly, these new findings have great relevance beyond wound healing itself. The injury repair process cuts across many disciplines, exte- ing to such broad fields as cancer, inflammation, and atherosclerosis. The relevance of the field to these many disciplines has generated great interest in models and methods for the study of wound healing. The goal of Wound Healing: Methods and Protocols is to provide scientists from many dis- plines with a compendium of classic and contemporary protocols from r- ognized experts in the field of wound healing. We hope this volume will be useful not only to those working within the field itself, but also to scientists from other disciplines who wish to adapt wound healing models to their own experimental needs. The process of wound healing encompasses many different biologic p- cesses, including epithelial growth and differentiation, fibrous tissue prod- tion and function, angiogenesis, and inflammation.

Inhaltsverzeichnis

Part I. Experimental Models of Wound Healing

A. In Vivo Animal Models
Excisional Wound Healing: An Experimental Approach
Stefan Frank and Heiko Kämpfer

Methods in Reepithelialization: A Porcine Model of Partial-Thickness Wounds
Heather N. Paddock, Gregory S. Schultz, and Bruce A. Mast

Incisional Wound Healing: Model and Analysis of Wound Breaking Strength
Richard L. Gamelli and Li-Ke He

Animal Models of Ischemic Wound Healing: Toward an Approximation of Human Chronic Cutaneous Ulcers in Rabbit and Rat
Mark Sisco and Thomas A. Mustoe

Corneal Injury: A Relatively Pure Model of Stromal-Epithelial Interactions in Wound Healing
Steven E. Wilson, Rahul R. Mohan, Renato Ambrosio, and Rajiv R. Mohan

Subcutaneous Sponge Models
David T. Efron and Adrian Barbul

A Mouse Model of Burn Wounding and Sepsis
Julia M. Stevenson, Richard L. Gamelli, and Ravi Shankar

A Porcine Burn Model
Adam J. Singer and Steve A. McClain

Wound Healing in Airways In Vivo
Steven R. White

Murine Models of Intestinal Anastomoses
David L. Williams and I. William Browder

Murine Model of Peritoneal Adhesion Formation
Andrew E. Jahoda, Mary Kay Olson, and Elizabeth J. Kovacs

Methods for Investigating Fetal Tissue Repair
Ziv M. Peled, Stephen M. Warren, Pierre J. Bouletreau, and Michael T. Longaker

Growth of Human Blood Vessels in Severe Combined Immunodeficient Mice: A New in Vivo Model System of Angiogenesis
Peter J. Polverini, Jacques E. Nör, Martin C. Peters, and David J. Mooney

B. Reviews of Specific Model Systems

Tissue Repair in Models of Diabetes Mellitus: A Review
David G. Greenhalgh

Wound Healing Studies in Transgenic and Knockout Mice: A Review
Richard Grose and Sabine Werner

Wound Repair in Aging: A Review
May J. Reed, TeruhikoKoike, and Pauli Puolakkainen

C. Human Wound Healing Models

Specimen Collection and Analysis: Burn Wounds
Areta Kowal-Vern and Barbara A. Latenser

Suction Blister Model of Wound Healing
Vesa Koivukangas and Aarne Oikarinen

Implantable Wound Healing Models and the Determination of Subcutaneous Collagen Deposition in Expanded Polytetrafluoroethylene Implants
Lars Nannestad Jorgensen, Søren Munk Madsen, and Finn Gottrup

D. In Vitro Models

The Fibroblast Populated Collagen Lattice: A Model of Fibroblast Collagen Interactions in Repair
H. Paul Ehrlich

A Quantifiable In Vitro Model to Assess Effects of PAI-1 Gene Targeting on Epithelial Cell Motility
Kirwin M. Providence, Lisa Staiano-Coico, and Paul J. Higgins

Human Skin Organ Culture
Ingrid Moll

In Vitro Matrigel Angiogenesis Model
Anna M. Szpaderska and Luisa A. DiPietro

Part II. Analysis and Manipulation of Wound Healing

Quantification of Wound Angiogenesis
Quentin E. H. Low and Luisa A. DiPietro

In Vivo Matrigel Migration and Angiogenesis Assays
Katherine M. Malinda

Endothelial Cell Migration Assay: A Quantitative Assay for Prediction of In Vivo Biology
Mark W. Lingen

Analysis of Collagen Synthesis
Robert F. Diegelmann

Method for Detection and Quantitation of Leukocytes During Wound Healing
Iulia Drugea and Aime L. Burns

Detection of Reactive Oxygen Intermediate Production by Macrophages
Jorge E. Albina and Jonathan S. Reichner

Measurement of Chemokines at the Protein Level in Tissue
Robert M. Strieter, Marie D. Burdick, John A. Belperio, and Michael P. Keane

Methods of Measuring Oxygen in Wounds
Harriet W. Hopf, Thomas K. Hunt, Heinz Scheuenstuhl, Judith M. West, Lisa M. Humphrey, and Mark D. Rollins.

Isolation, Culture, and

Pressestimmen

From the reviews:

"Clinical and basic science researchers with an interest in wound healing of various kinds are an appropriate audience for this work. Editors and authors represent some of the leading wound healing laboratories in the United States with a smaller number of presentations from overseas. . .Chapters are written in a straightforward manner. . .This book is a collection of techniques and insights which belongs on the shelf of laboratories or research organizations having an interest in wounds." -Doody's Health Sciences Book Review Journal

"This informative volume provides a highly practical collection of widely used model systems and methods for studying the injury repair process and is therefore highly recommended as a valuable reference source for all individuals with interests in wound healing." - Carbohydrate Polymers

"The editors have done an impressive job of editing the book into both a practical and readable style. It is highly recommended for any group that wishes to participate in this exciting and expanding field of research." -British Journal of Surgery

"This book ... provides a range of classical and more recent tools in wound research. ... an impressive collection of both animal and in vitro-based techniques useful for experimental studies of tissue damage and repair. ... Each chapter has a concluding notes section offering valuable suggestions and warnings based on practical experience. The book is highly recommended for all basic and clinical researchers involved in tissue repair biology and particularly those with an interest in experimental wound modelling and analysis." (Ola Rollman, Acta Dermato-Venereologica, Vol. 85, 2005)
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