Ancient DNA Typing is a comprehensive manual about the analysis of ancient and degraded DNA. It is an equally useful textbook and working tool for various disciplines such as: Anthropology, Archaeology, Forensic Medicine, Conservation Biology, Environmental Sciences and Food Engineering.
The reader benefits from a clear division of strategies, methods and applications with many interesting examples of the analysis of historic skeletal and cultural remains. The book contains a detailed protocol chapter which serves as a lab manual with the most important methods for DNA extraction, PCR and electrophoretic analysis of degraded samples. The appendix helps to set up a PCR lab specializing in ancient DNA research and answers the most frequent questions concerning technical problems in investigations of degraded DNA.
Inhaltsverzeichnis
1 Introduction. - 2 DNA markers. - 3 aDNA extraction. - 4 PCR. - 5 Analysis of PCR products. - 6 Authenticity of results. - 7 Applications. - 8 Protocols. - I Laboratory equipment. - I. 1 Pre-PCR laboratory equipment. - I. 2 PCR laboratory. - I. 3 Post-PCR laboratory. - I. 4 Photographic documentation. - II PCR trouble shooting. - III Internet sites of interest.