Molecular Endocrinology

In this volume, leaders in the field share a diversity of cutting-edge techniques in endocrinology. Coverage includes protocols that allow investigators at all stages of their scientific career to successfully perform these techniques.

Determining the Affinity of Hormone? Receptor Interaction. - Determination of Serum Estradiol Levels by Radiometric and Chemiluminescent Techniques. - Identification of Natural Human Glucocorticoid Receptor (hGR) Mutations or Polymorphisms and Their Functional Consequences at the Hormone Receptor Interaction Level. - Monitoring Insulin-Stimulated Production of Signaling Lipids at the Plasma Membrane. - Gene Expression Profiling in the Aging Ovary. - Genomics Analysis: Endometrium. - Detection of Ovarian Matrix Metalloproteinase mRNAs by In Situ Hybridization. - Adenoviral Gene Transfer into Isolated Pancreatic Islets. - Basic Molecular Techniques for the Detection of Single Nucleotide Polymorphisms: Genome-Wide Applications in Search for Endocrine Tumor Related Genes. - Methylated DNA Immunoprecipitation and Microarray-Based Analysis: Detection of DNA Methylation in Breast Cancer Cell Lines. - Use of Reporter Genes to Study the Activity of Promoters in Ovarian Granulosa Cells. - Use of Reporter Genes to Study Promoters of the Androgen Receptor. - Isolation of Proteins Associated with the DNA-Bound Estrogen Receptor ? . - Chromosome-Wide Analysis of Protein Binding and Modifications. - Genome-Wide Analysis for Protein? DNA Interaction: ChIP-Chip. - Detection of ER? -SRC-1 Interactions Using Bioluminescent Resonance Energy Transfer. - Detection of Proteins Sumoylated In Vivo and In Vitro. - Identification of Alternative Transcripts Using Rapid Amplification of cDNA Ends (RACE). - Use of Laser Capture Microdissection in Studying Hormone-Dependent Diseases: Endometriosis. - Reporter Mice for the Study of Intracellular Receptor Activity. - Real-Time Non-invasive Imaging of ES Cell-Derived Insulin Producing Cells. - Transgenic Mouse Technology: Principles and Methods. - Breast Tumor-InitiatingCells Isolated from Patient Core Biopsies for Study of Hormone Action. - Markers of Oxidative Stress and Sperm Chromatin Integrity. - Planning and Executing a Genome Wide Association Study (GWAS).