Drosophila

This second edition volume expands on the previous edition by presenting updated protocols for several of the techniques described in the first edition of Drosophila: Methods and Protocols and current methods that cover recent breakthroughs in Drosophila research. The book begins with a description of FlyBase--a database of genes and genomes--followed by the presentation of systems for versatile gene expression in the fly. The first few chapters in this book detail gene knockdown and editing, including CRISPR-Cas9 and protein knockdown. The next few chapters are devoted to methods describing live imaging of different tissues and organs, followed by chapters on how to quantify image data and how to probe tissue mechanics by laser ablation. The next two chapters provide methods for analyzing transcription followed by protocols to study growth, metabolism, ageing, and behavior in Drosophila. This volume concludes with chapters on electrophysiological recordings and methods to establish cell lines. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Drosophila: Methods and Protocols, Second Edition is a valuable source of hands-on protocols and reviews for molecular, cell, and developmental biologists using Drosophila as model systems in their work.

Using FlyBase: A Database of
Drosophila
Genes and Genomes. - The GAL4 System: A Versatile System for Manipulation and Analysis of Gene Expression. - The Q-System: A Versatile Expression System for
Drosophila. -
Analysis of MicroRNA Function in
Drosophila. -
Methods for High-Throughput RNAi screening in
Drosophila
Cells. - A Guide to Genome-Wide
In Vivo
RNAi Applications in
Drosophila. -
Creating Heritable Mutations in
Drosophila
with CRISPR-Cas9. - Performing Chromophore-Assisted Laser Inactivation in
Drosophila
Embryos Using GFP. - deGradFP: A System to Knockdown GFP-Tagged Proteins. - Sample Preparation and Mounting of
Drosophila
Embryos for Multi-View Light Sheet Microscopy. - Cultivation and Live-Imaging of
Drosophila
Imaginal Discs. - Cultivation and Live Imaging of
Drosophila
Ovaries. - Segmentation and Quantitative Analysis of Epithelial Tissues. - Laser Ablation to Probe the Epithelial Mechanics in
Drosophila. -
Rapid Ovary Mass-isolation (ROMi) to Obtain Large Quantities of
Drosophila
Egg Chambers for Fluorescent In-Situ Hybridization. - Chromatin Immunoprecipitation for Analyzing Transcription Factor Binding and Histone Modifications in
Drosophila. -
Protocols to Study Growth and Metabolism in
Drosophila. -
Protocols to Study Ageing in
Drosophila. -
Protocols to Study Behavior in
Drosophila. -
Electrophysiological Recordings from Lobula Plate Tangential Cells in
Drosophila. -
Methods to Establish a
Drosophila
Cell Line.