Exocytosis and Endocytosis

An Overview of Methods for Analyzing Vesicular Trafficking. - Vesicular Trafficking: Molecular Tools and Targets. - Pharmacological Inhibition of Endocytic Pathways: Is It Specific Enough to Be Useful? . - Cell-Free and Biochemical Assays of Exocytosis and Endocytosis. - In Vitro Assays to Measure SNARE-Mediated Vesicle Fusion. - Imaging Ca2+-Triggered Exocytosis of Single Secretory Granules on Plasma Membrane Lawns from Neuroendocrine Cells. - FRAP Analysis of Secretory Granule Lipids and Proteins in the Sea Urchin Egg. - A Cell-Free Assay for Endocytosis of E-Cadherin. - Cell-Surface Biotinylation to Study Endocytosis and Recycling of Occludin. - Fractionation of Subcellular Membrane Vesicles of Epithelial and Nonepithelial Cells by OptiPrep Density Gradient Ultracentrifugation. - Identification and Characterization of Interacting Partners of Rab GTPases by Yeast Two-Hybrid Analyses. - Analysis of Endocytosis and Exocytosis in Different Cell Systems. - High-Throughput Analysis of the Dynamics of Recycling Cell Surface Proteins. - Studying Phagocytosis by Live-Cell Scintillation Proximity Assay. - Transcytosis of Polymeric Immunoglobulin A in Polarized Madin Darby Canine Kidney Cells. - The Use of Syntaxin Chimeras to Study Polarized Protein Trafficking in Epithelial Cells. - Targeting the Epithelial SNARE Machinery by Bacterial Neurotoxins. - Exocytosis of Endothelial Cells Is Regulated by N-Ethylmaleimide-Sensitive Factor. - Molecular Dissection of HCl Secretion in Gastric Parietal Cells Using Streptolysin O Permeabilization. - Addressing Membrane Protein Topology Using the Fluorescence Protease Protection (FPP) Assay. - Visualizing Clathrin-Mediated IgE Receptor Internalization by Electron and Atomic Force Microscopy. - Analyzing Endosomes in Nonsectioned Cells by TransmissionElectron Microscopy. - Imaging Exocytosis of Single Insulin Secretory Granules With TIRF Microscopy. - Reconstitution of Depolarization and Ca2+-Evoked Secretion in Xenopus Oocytes Monitored by Membrane Capacitance. - Low-Noise Recording of Single-Vesicle Capacitance Steps in Cell-Attached Patches. - Good Practices in Single-Cell Amperometry. - Analysis of Single-Vesicle Exocytotic Events Recorded by Amperometry. - Analysis of Exocytosis and Endocytosis in Model Organisms. - Application of RNAi Technology and Fluorescent Protein Markers to Study Membrane Traffic in Caenorhabditis elegans. - FM 1-43 Labeling of Synaptic Vesicle Pools at the Drosophila Neuromuscular Junction. - Probing E-Cadherin Endocytosis by Morpholino-Mediated Rab5 Knockdown in Zebrafish. - Quantifying Endocytosis In Vivo Using Intravital Two-Photon Microscopy.