This is the first book that comprehensively and systematically describes the new technology of hydrophilic interaction liquid chromatography (HILIC). Hydrophilic interaction chromatography is a separation technique suitable for polar and hydrophilic compounds and orthogonal to reversed phase liquid chromatography. From small organic molecules to proteins, the text explores the many applications of HILIC in the analytical field. Winner of the President's Award for Excellence, the author explains how HILIC can significantly improve analytical throughput by shortening sample preparation procedure, which is one of the bottlenecks for drug discovery and development in the pharmaceutical industry.
Inhaltsverzeichnis
Aqueous Normal-Phase Chromatography: The Bridge between Reversed-Phase and HILIC. Application of the Calculated Physicochemical Property, Log D, to Hydrophilic Interaction Chromatography in the Bioanalytical Laboratory. HILIC Behavior of Reversed-Phase/Ion-Exchange Mixed-Mode Stationary Phases and Their Applications. Mechanisms Controlling the Separation and Retention of Proanthocyanidins and Other Plant Polyphenols in Hydrophilic Interaction Chromatography. Applications of Hydrophilic Interaction Chromatography to Food Analysis. Hydrophilic Interaction Liquid Chromatography-Mass Spectrometry (HILIC-MS) of Paralytic Shellfish Poisoning Toxins, Domoic Acid, and Assorted Cyanobacterial Toxins. Application of HILIC for Polar Environmental Contaminants (Including Pharmaceuticals) in Aquatic Systems. Hydrophilic Interaction Chromatography-Mass Spectrometry: An Environmental Application. Clinical Applications of Hydrophilic Interaction Liquid Chromatography. Dimethylarginine Analysis by HILIC-MS/ MS and Its Applications in Clinical Chemistry and In Vivo Animal Models. Polar Functional Groups for HILIC Method. Analysis of Pharmaceutical Impurities Using Hydrophilic Interaction Liquid Chromatography. Fast In-Process Method for the Determination of Ioversol and Related Polar Compounds by Hydrophilic Interaction Chromatography (HILIC) and UPLC. Retention Behavior of Hydrazine Derivatives in HILIC Mode. HILIC Retention Behavior and Method Development for Highly Polar Basic Compounds Used in Pharmaceutical Synthesis. Retention of Polar Acidic and Basic Drugs by HILIC. Retention and Selectivity of Polar Stationary Phases for Hydrophilic Interaction Chromatography. HILIC-MS/MS for the Determination of Polar Bioactive Substances: Representative Applications in the Fields of Pharmacokinetics and Metabolic Profiling. Method Development and Analysis of Mono- and Diphosphorylated Nucleotides by HILIC HPLC-ESI-MS. Hydrophilic Interaction Liquid Chromatography-Based Enrichme
