Enzyme Engineering

Written by one of the pioneers, this book presents enzyme engineering and its applications at its best. Beside established topics like enzyme activity and selectivity, emerging fields like machine learning and AI are presented, too.

Introduction to Directed Evolution and Rational Design as Protein Engineering Techniques
-Methods and Aims of Directed Enzyme Evolution
-Short History of Directed Enzyme Evolution
-Methods and Aims of Rational Design of Enzymes
Screening and Selection Techniques
Gene Mutagenesis Methods
Guidelines for Applying Gene Mutagenesis Methods in Organic Chemistry, Pharmaceutical Applications and Biotechnology
Case Studies of Protein Engineering of Activity and Selectivity
-Epoxide Hydrolase
-Transaminase as an Industrial Example with Pharmaceutical Application
-Geranylgeranyl Diphosphate Synthase for Efficient Carotenoid Production
-Cytochrome P450 Monooxygenases for Synthesis of Hydroxylation of Steroids Needed in the Preparation of Pharmaceuticals
-Lipase for Stereocomplementary Production of Organic Compounds with Two Chirality Centers
-Further Examples Using Other Enzyme Types
Protein Engineering of Enzyme Robustness
-Examples of Relevance to Organic and Pharmaceutical Applications
-Examples of Relevance to Biotechnology
Artificial Metallo-Enzymes for Promiscuous Transformations Using Known Organic Reaction Types as a Guide
Learning Lessons from Protein Engineering
Perspectives for Future Work
-In Extending Applications in Organic and Pharmaceutical Chemistry
-In Extending Biotechnological Contributions to Ecology