This volume will be of interest to epidemiologists, food microbiologists, and anyone working on comparing bacterial isolates. Pulse Field Gel Electrophoresis: Methods and Protocols guides readers through methods and protocols that will advance the harmonisation of PFGE methodologies and facilitate inter-laboratory comparisons of PFGE profiles from pathogenic and non-pathogenic bacteria. As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Concise and easy-to-use, Pulse Field Gel Electrophoresis: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Inhaltsverzeichnis
Pulsed-field Gel Electrophoresis for Disease Monitoring and Control. - Harmonization of PFGE Profile Analysis by using Bioinformatics Tools: Example of the Listeria monocytogenes European Union Reference Laboratory Network. - PFGE as a Tool to Track Listeria monocytogenes in Food Processing Facilities: Case Studies. - Case Study of the use of Pulsed Field Gel Electrophoresis in the Detection of a Food-borne Outbreak. - Pulsed-Field Gel Electrophoresis for Listeria monocytogenes. - Pulsed-field Gel Electrophoresis (PFGE) for Pathogenic Cronobacter Species. - Pulsed-Field Gel Electrophoresis of Bacillus cereus Group Strains. - Pulsed-Field Gel Electrophoresis of Staphylococcus aureus. - The Use of Pulsed-Field Gel Electrophoresis for Genotyping of Clostridium difficile. - Molecular Subtyping of Clostridium botulinum by Pulsed-Field Gel Electrophoresis. - Pulsed Field Gel Electrophoresis of Yersinia pestis. - Pulsed Field Gel Electrophoresis of Group A streptococci. - Application of Pulsed Field Gel Electrophoresis to Type Campylobacter jejuni. - Pulsed-field Gel Electrophoresis of Pseudomonas aeruginosa. - PFGE for Shiga toxin-producing Escherichia coli O157:H7 (STEC O157) and non-O157 STEC. - Pulsed Field Gel Electrophoresis (PFGE) of Salmonella enterica. - PFGE Protocols to Distinguish Subspecies ofLactococcus lactis. - The use of PFGE Method in Genotyping of Selected Bacteria Species of the Lactobacillus genus. - Pulsed Field Gel Electrophoresis for Leuconostoc mesenteroides and pseudomesenteroides. - Pulsed Field Gel Electrophoresis for Bifidobacterium. - Pulsed Field Gel Electrophoresis for Dairy Propionibacteria.
