Salmonella

Salmonella: Methods and Protocols, Second Edition expands upon the previous edition with current, detailed methods on different aspects and landmarks advancing knowledge on salmonella research. With new chapters on molecular assays for detection, identification and serotyping of salmonella, quantitative proteomic identification of host factors involved in salmonella infection, determination of antimicrobial resistance in salmonella, site-directed mutagenesis, chromosomal gene analysis, development of bacterial nanoparticle vaccine, attachment of nanoparticle cargo to biotinylated salmonella for combination bacteriotherapy against tumors, various microscopy methods to analyze salmonella interaction with host cells, in vitro modeling of gallbladder-associated salmonella colonization, and analysis of salmonella phages and prophages. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Salmonella: Methods and Protocols, Second Edition present methods that are of value to investigators in the salmonella field.

Luminex
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Multiplex Bead Suspension Arrays for the Detection and Serotyping of
Salmonella
spp. . - Quantitative Proteomic Identification of Host Factors Involved in the
Salmonella typhimurium
Infection Cycle. - Determination of Antimicrobial Resistance in
Salmonella
spp. . - Red-mediated Recombineering of
Salmonella enterica
Genomes. - A Method to Introduce an Internal Tag Sequence into a
Salmonella
Chromosomal Gene. - Generation and use of Site-directed Chromosomal
cyaA
Translational Fusions in
Salmonella enteric. -
Detection of Antimicrobial (Poly)Peptides with Acid Urea Polyacrylamide Gel Electrophoresis followed by Western Immunoblot. - Detecting non-typhoid
Salmonella
in Humans by Enzyme-linked

Immunosorbent Assays (ELISAs): Practical and Epidemiological Aspects. - Study of the Stn Protein in
Salmonella
; A Regulator of Membrane Composition and Integrity. - Development of a Bacterial Nanoparticle Vaccine. - Direct Attachment of Nanoparticle Cargo to
Salmonella typhimurium
Membranes Designed for Combination Bacteriotherapy Against Tumors. - Applications of Microscopy in
Salmonella
Research. - Live Cell Imaging of Intracellular
Salmonella enteric. -
In vitro Modeling of Gallbladder-associated
Salmonella
spp. Colonization. -
Salmonella
Phages and Prophages Genomics, Taxonomy, and Applied Aspects.