Unconventional Protein Secretion

This volume presents relevantbackground information to understanding the molecular basis governingunconventional protein secretion (UPS), and in particular explores the latesttechniques and protocols that have been successfully applied for the study ofthis topic. Detailed chapters include an overview of conventional andunconventional secretory pathways along with multidisciplinary approaches andmethods used for UPS analysis in different organisms. Written in the highly successful Methods inMolecular Biology series format,chapters include introductions to theirrespective topics, lists of the necessary materials and reagents, step-by-step,readily reproducible laboratory protocols, and tips on troubleshooting andavoiding known pitfalls.

Authoritative and cutting-edge, UnconventionalProtein Secretion: Methods and Protocols will beusefulfor all interested in the secretory pathway field as well as applications incell biology, cell development, biomedical research, and healthcare.

ER to Golgi Dependent Protein Secretion: The Conventional Pathway.- Unconventional
Protein Secretion in Animal Cells.- Unconventional
Protein Secretion in Plants.- Chemical
Secretory Pathway Modulation in Plant Protoplasts.- From Cytosol to the Apoplast: The Hygromycin
Phosphotransferase (HYG
^R
) Model in Arabidopsis.- Following the Time-Course of Post-Pollination Events by Transmission Electron
Microscopy (TEM): Buildup of Exosome-Like Structures with Compatible
Pollinations.- Investigating Alternative
Transport of Integral Plasma Membrane Proteins from the ER to the Golgi:
Lessons from the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR).- Quantification of a Non-Conventional Protein Secretion:
The Low Molecular Weight FGF-2 Example.- Human
Primary Keratinocytes as a Tool for the Analysis of Caspase-1-Dependent.- A Reporter System to Study Unconventional Secretion of Proteins Avoiding
N
-glycosylation in
Ustilago maydis.-
Stress-inducible Protein 1 (STI1): Extracellular Vesicles Analysis And
Quantification.- Analysis of Yeast Extracellular Vesicles.- Exploring the
Leishmania

Hydrophilic Acylated Surface Protein B (HASPB) Export Pathway by Live Cell
Imaging Methods.- Characterization of the Unconventional Secretion of the Ebola Matrix
Protein VP40.- Role and
Characterization of synuclein- Unconventional Protein Secretion in Cancer
Cells.- Characterization of the Tumor Secretome from Tumor Interstitial Fluid (TIF).- Vacuum Infiltration-Centrifugation Method for Apoplastic Protein Extraction in
Grapevin.- Isolation of Exosome-Like Vesicles from Plants by Ultracentrifugation on
Sucrose/Deuterium Oxide (D
₂
O) Density Cushions.