Brassinosteroids

Protocol for Extraction and Isolation of Brassinosteroids from Plant Tissues. - Synthetic Protocol for AFCS: A Biologically Active Fluorescent Castasterone Analog Conjugated to an Alexa Fluor 647 Dye. - Physiological Analysis of Brassinosteroid Responses and Sensitivity in Rice. - Light Regulation of Brassinosteroid Signaling Components: Checking Regulation of Protein Stability in Darkness. - Approaches to Study Light Effects on Brassinosteroid Sensitivity. - A Technical Framework for Studying the Signaling Nexus of Brassinosteroids and Immunity. - Identification of Brassinosteroid Target Genes by Chromatin Immunoprecipitation Followed by High-Throughput Sequencing (ChIP-seq) and RNA-Sequencing. - Quantitation of Cell Type-Specific Responses to Brassinosteroid by Deep Sequencing of Polysome-Associated Polyadenylated RNA. - Methods for Modeling Brassinosteroid-Mediated Signaling in Plant Development. - Quantitative Microscopic Analysis of Plasma Membrane Receptor Dynamics in Living Plant Cells. - Analysis of In Vitro DNA Interactions of Brassinosteroid-Controlled Transcription Factors Using Electrophoretic Mobility Shift Assay. - Identification of Brassinosteroid Signaling Complexes by Coimmunoprecipitation and Mass Spectrometry. - Simplified Enrichment of Plasma Membrane Proteins from Arabidopsis thaliana Seedlings Using Differential Centrifugation and Brij-58 Treatment. - Probing Activation and Deactivation of the BRASSINOSTEROID INSENSITIVE1 Receptor Kinase by Immunoprecipitation. - The Primary Root of Sorghum bicolor (L. Moench) as a Model System to Study Brassinosteroid Signaling in Crops. - Brassinosteroid Action in Plant Abiotic Stress Tolerance.