Cryptococcus neoformans

This volume explores the latest developments in the study of Cryptococcus neoformans and its pathology, along with discussion on newly used therapeutics. The chapters in this book cover a wide range of protocols commonly used in Cryptococcus research such as animal models, genetics techniques, virulence factor phenotyping, and microscopy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Cryptococcus neoformans: Methods and Protocols is a valuable resource that will help researchers further enhance their understanding of this pathogenic yeast, and will aid in new discoveries and therapeutics.

A
Galleria
Model of
Cryptococcus neoformans
Infection. - The Rabbit Model of Cryptococcal Meningitis. - Models for Inducing Experimental Cryptococcosis in Mice. - In Vivo Modeling of
Cryptococcus neoformans
Infection and Collection of Murine Samples. - Biolistic Transformation of
Cryptococcus neoformans. -
Genetic Transformation of
Cryptococcus
Species with
Agrobacterium
Transfer DNA. - Gene Silencing via RNA Interference in
Cryptococcus. - Cryptococcus neoformans
Transcriptome Analysis to Identify Differentially Expressed Genes Using the STAR Pipeline in CyVerse Discovery Environment. - Proteomic Profiling of Samples Derived from a Murine Model Following
Cryptococcus neoformans
Infection. - Electron Microscopy of
Cryptococcus neoformans
: Processing Challenges to Avoid Artifacts Glauber R. de S. Araújo, Bruno Pontes, and Susana Frases. - Assessing Phagocytosis of
Cryptococcus neoformans
Cells in Human Monocytes or the J774 Murine Macrophage Cell Line. - Interaction Between Macrophages and
Cryptococcus neoformans:
Distinguishing Phagocytosed Versus External Fungi. - Detection and Quantification of
Cryptococcus
Uptake by Phagocytic Cells Using Imaging Flow Cytometry. - Two Methods of Measuring
Cryptococcus neoformans
Fungal Burden in Macrophages. - Quantification of
C. neoformans
Capsule Diameter. - Methods of Cryptococcal Polysaccharide Analysis Using ELISA. - Measuring Laccase Activity and Melanin Production in
Cryptococcus neoformans. -
Measuring Urease and Phospholipase Secretion in
Cryptococcus neoformans. -
Measuring Stress Phenotypes in
Cryptococcus neoformans. -
Antibody Isolation in
C. neoformans. -
Fluorescence and Biochemical Assessment of the Chitin and Chitosan Content of
Cryptococcus. -
Induction of Dormancy in
Cryptococcus neoformans
In Vitro: The HypNOS Protocol. - Analysis of
Cryptococcus
Extracellular Vesicles. - Preparation of Biologically Active Fractions Enriched with Glucuronoxylomannan, the Main Antigen of the Cryptococcal Capsule. - Measuring Replicative Lifespan in
Cryptococcus neoformans. -
In Vitro Titan Cell Generation in
Cryptococcus neoformans
and Automated Cell Size Measurements. - Chitosan-Deficient
Cryptococcus
as Whole-Cell Vaccines. - Vaccine Strategies for
Cryptococcus neoformans.