This volume focuses on the latest techniques used in forensic DNA analysis. The chapters include a comprehensive collection of extraction, quantification, STR amplification, and detection methods for routine forensic samples, including manual, semi-automated, and automated procedures using both home-brew and commercial products. The chapters also discuss probabilistic modeling software and specialized start-to-finish procedures for mitochondrial DNA analysis, archived latent fingerprints, latent DNA, rapid DNA profiling, and next-generation sequencing. Written in the highly successful Methods in Molecular Biology series format, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and practical, Forensic DNA Analysis: Methods and Protocols is a valuable resource for researchers interested in learning more about forensic DNA analysis procedures.
Inhaltsverzeichnis
Forensic DNA Analysis: An Overview of the Laboratory Process. - Organic Extraction of Nucleic Acids using Ethanol Precipitation or Microcon® Centrifugal Filter Purification Methods. - Manual Silica-Based DNA Extractions. - Applied Biosystems PrepFiler Forensic DNA Extraction Kit (Manual and Semi-Automated via AutoMate Express ). - Robotic DNA Extraction Utilizing Qiagen BioSprint® 96 Workstation. - DNA Extraction of Bone through Demineralization. - Differential Extraction with Purification via Organic/Microcon® and Promega DNA IQ Methods. - DNA Purification from Bloodstains and Buccal Cells/Saliva on FTA® Cards. - Yield Gel via Quantitative Gel Electrophoresis. - Quantitative PCR of Alu Repeats using PowerUp SYBR® Green Master Mix. - Quantitation of DNA using the Applied Biosystems Quantifiler® Trio DNA Quantification Kit. - QIAGEN' s Investigator® Quantiplex Pro Kit. - DNA Amplification using Promega' s PowerPlex® Fusion Systems (5C and 6C). - Amplification of Extracted DNA and Direct Amplification with the PowerPlex® Y23 System. - Applied Biosystems' GlobalFiler PCR Amplification Kit. - QIAGEN s Investigator® 24plex QS and GO! PCR Amplification. - Low Volume STR Amplification Options: Coupling with Standard or Fast PCR, Traditional or Normalized DNA Extraction, and/or Traditional or Alternative Capillary Electrophoresis. - Capillary Electrophoresis with Applied Biosystems' 3500 Genetic Analyzer. - Likelihood Ratio Calculation using LRmix Studio. - Mitochondrial DNA Analysis. - An Optimized Forensic DNA Analysis Workflow for Obtaining STR Results from Archived Latent Fingerprints. - Detection of Latent DNA using a DNA Binding Dye. - Rapid DNA Profile Development with Applied Biosystems RapidHIT ID System. - Next-Generation Sequencing: ForenSeq DNA Signature Prep Kit with the Illumina MiSeq FGx.
