Proteomic analyses have proven to be an invaluable tool in obtaining high-throughput protein identification from low-abundance, complex biological samples. In Organelle Proteomics, detailed protocols provide step-by-step instructions to successfully study organelle proteomes by performing the purification of the various organelles present in eukaryotic cells, as well as by preparing certain sub-fractions of organelles. A series of chapters cover the whole analytical procedure of organelle characterization, from its purification starting with whole cells up to protein identification using mass spectrometry. Devoted to methods enabling a global estimate of the reliability of the protein list assigned to an organelle, Organelle Proteomics allows scientists to gain a vital and important understanding of organelle study.
Inhaltsverzeichnis
Analysis of Organelles by On-Line Two-Dimensional Liquid Chromatography Tandem Mass Spectrometry. - Analysis of Organelles by On-Line Two-Dimensional Liquid Chromatography Tandem Mass Spectrometry. - Analysis of Organelles, Vesicles, and Protein Machineries. - Purification and Proteomic Analysis of Chloroplasts and their Sub-Organellar Compartments. - Proteomic Analysis of the Stacked Golgi Complex. - Purification of Saccharomyces cerevisiae Mitochondria by Zone Electrophoresis in a Free Flow Device. - Preparation of Respiratory Chain Complexes from Saccharomyces cerevisiae Wild-Type and Mutant Mitochondria. - Mitochondrial Proteomics. - Purification and Proteomic Analysis of the Mouse Liver Mitochondrial Inner Membrane. - Subcellular Fractionation and Proteomics of Nuclear Envelopes. - Purification and Proteomic Analysis of a Nuclear-Insoluble Protein Fraction. - Preparation Methods of Human Metaphase Chromosomes for their Proteome Analysis. - Purification and Proteomic Analysis of Plant Plasma Membranes. - Protocol to Enrich and Analyze Plasma Membrane Proteins from Frozen Tissues. - Proteomic Characterization of Membrane Protein Topology. - Free Flow Isoelectric Focusing. - Use of Gas-Phase Fractionation to Increase Protein Identifications. - Purification and Proteomic Analysis of Lysosomal Integral Membrane Proteins. - Affinity Purification of Soluble Lysosomal Proteins for Mass Spectrometric Identification. - Purification and Proteomic Analysis of Synaptic Vesicles. - Purification and Proteomics Analysis of Pancreatic Zymogen Granule Membranes. - Isolation and Proteomic Analysis of Chlamydomonas Centrioles. - Purification and Proteomic Analysis of 20S Proteasomes from Human Cells. - Characterization of E. coli Ribosomal Particles. - Cellular, Genetic and Molecular Mechanisms of PlantEmbryogenesis. - Assessment of Organelle Purity Using Antibodies and Specific Assays. - Identifying Bona Fide Components of an Organelle by Isotope-Coded Labeling of Subcellular Fractions. - Determination of Genuine Residents of Plant Endomembrane Organelles using Isotope Tagging and Multivariate Statistics. - Quantitative Proteomic Analysis to Profile Dynamic Changes in the Spatial Distribution of Cellular Proteins. - Use of Transcriptomic Data to Support Organelle Proteomic Analysis.
