Organelle Proteomics

Inhaltsverzeichnis

1. - Analysis of organelles by on-line two-dimensional liquid chromatography - tandem mass spectrometry (2DLC-MS/MS) Edwin P. Romijn and John R. Yates III. Analysis of organelles, vesicles and protein machineries 2 - Purification and proteomic analysis of chloroplasts and their sub-organellar compartments

Daniel Salvi, Norbert Rolland, Jacques Joyard and Myriam Ferro 3 - Proteomic analysis of the stacked Golgi complex

Mark S. Lowenthal, Kathryn E. Howell, and Christine C. Wu 4 - Purification of Saccharomyces cerevisiae mitochondria by zone-electrophoresis in a free flow device (ZE-FFE)

Hans Zischka, Norbert Kinkl, Ralf J. Braun and Marius Ueffing 5 - Preparation of Respiratory Chain Complexes from Saccharomyces cerevisiae Wild Type and Mutant Mitochondria: Activity Measurement and Subunit Composition Analysis

Claire Lemaire and Geneviève Dujardin 6 - Mitochondrial proteomics: Analysis of a whole mitochondrial extract with two-dimensional electrophoresis Thierry Rabilloud. 7 - Purification and proteomic analysis of the mouse liver mitochondrial inner membrane

Sandrine Da Cruz and Jean-Claude Martinou 8 - Subcellular Fractionation and Proteomics of Nuclear Envelopes

Laurence Florens, Nadia Korfali and Eric C. Schirmer. 9 - Purification and proteomic analysis of the nuclear insoluble protein fraction

Tsuneyoshi Horigome, Kazuhiro Furukawa and Kohei Ishii 10 - Preparation Methods of Human Metaphase Chromosomes for Their Proteome Analysis.

Kiichi Fukui and Hideaki Takata 11 - Purification and proteomic analysis of plant plasma membranes

Erik Alexandersson, Niklas Gustavsson, Katja Bernfur, Adine Karlsson, Per Kjellbom and Christer Larsson 12 - Protocol to enrich and analyze plasma membrane proteins from frozen tissue

Jacek R. Wisniewski 13 - Proteomic characterization of membrane proteintopology

Adele R. Blackler and Christine C. Wu 14 - Free Flow Isoelectric Focussing (FF-IEF): A method for the separation of both hydrophilic and hydrophobic proteins of rat liver peroxisomes

Markus Islinger and Gerhard Weber 15 - Use of gas-phase-fractionation to increase protein identifications: application to the peroxisome

Jacob Kennedy and Eugene C. Yi 16 - Purification and proteomic analysis of lysosomal integral membrane proteins

Huiwen Zhang, Xiaolian Fan, Rick Bagshaw, Don J. Mahuran, John W. Callahan. 17 - Affinity purification of soluble lysosomal proteins for mass spectrometric identification

Sylvie Kieffer-Jaquinod, Agnès Chapel, Jérôme Garin and Agnès Journet 18 - Purification and proteomic analysis of synaptic vesicles

Holly D. Cox and Charles M. Thompson. 19 - Purification and proteomic analysis of pancreatic zymogen granule membranes

Xuequn Chen and Philip C. Andrews 20 - Isolation and proteomic analysis of Chlamydomonas centrioles

Lani C. Keller and Wallace F. Marshall. 21 - Purification and proteomic analysis of 20S proteasomes from human cells

Marie-Pierre Bousquet-Dubouch, Sandrine Uttenweiler-Joseph, Manuelle Ducoux-Petit, Mariette Matondo, Bernard Monsarrat, and Odile Burlet-Schiltz. 22 - Characterization of E. coli ribosomal particles: combined analysis of whole proteins by mass spectrometry and of proteolytic digests by liquid chromatography - tandem mass spectrometry.

Isabelle Iost, Julie Charollais, Joëlle Vinh and Delphine Pflieger Proteins identified in the organelle sample: true components or contaminants? 23 - Assessment of organelle purity using antibodies and specific assays: the example of the chloroplast envelope

Daniel Salvi, Norbert Rolland, Jacques Joyard and Myriam Ferro 24 - Identifying bona fide components of an organelle by isotope coded labelling of