Bioluminescent Imaging

This volume provides detailed protocols and methodologies required to perform bioluminescent imaging in multiple stages, enabling the reader to integrate this technology into their laboratory-based imaging experiments. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Bioluminescent Imaging: Methods and Protocols to ensure successful results in the further study of this vital field.

High-throughput Bioluminescence Imaging and Reporter Gene Assay with 3D Spheroids from Human Cell Lines. - Bioluminescence Resonance Energy Transfer (BRET) Coupled Near-Infrared Imaging of Apoptotic Cells. - High-throughput Analysis of Endocrine Disrupting Compounds using BLYES and BLYAS Bioluminescent Yeast Bioassays. - Bioluminescent Imaging of Single Bacterial Cells Using an Enhanced
ilux
Operon. - Bioluminescent Imaging and Tracking of Bacterial Transport in Soils. -
In vivo
Bioluminescent Imaging of
Yersinia ruckeri
Pathogenesis in Fish. -
In Vivo
Bioluminescent Monitoring of Parasites in BALB/c Mouse Models of Cutaneous Leishmaniasis Drug Discovery. - Multiplex Imaging of Polymicrobial Communities Murine Models to Study Oral Microbiome Interactions. - Bioluminescence Imaging to Study Mature Biofilm Formation by
Candida spp.
and Antifungal Activity
in vitro
and
in vivo. -
Bioluminescence Imaging of Neuroinflammation in a Mouse Model of Parkinson s Disease. - Continual Conscious Bioluminescent Imaging in Freely Moving Mice. - In Vivo Bioluminescent Imaging of Marburg Virus in a Rodent Model. - Continuous and Real-time
In Vivo
Autobioluminescent Imaging in a Mouse Model. -
In vivo
Tracking of Tumor-derived Bioluminescent Extracellular Vesicles in Mice. - Bioluminescence Imaging in the Chick Chorioallantoic Membrane Assay. - Application of a cybLuc Aminoluciferin for Deep Tissue Bioluminescence Imaging in Rodent Models. - The Application of Single Cell Bioluminescent Imaging to Monitor Circadian Rhythms of Individual Plant Cells.