Most research in the life sciences involves a core set of
molecular-based equipment and methods, for which there is no
shortage of step-by-step protocols. Nonetheless, there remains an
exceedingly high number of inquiries placed to commercial technical
support groups, especially regarding problems.
Molecular Biology Problem Solver: A Laboratory
Guide asks the reader to consider crucial questions, such
as:
* Have you selected the most appropriate research strategy?
* Have you identified the issues critical to your successful
application of a technique?
* Are you familiar with the limitations of a given
technique?
* When should common procedural rules of thumb not be
applied?
* What strategies could you apply to resolve a problem?
A unique question-based format reviews common assumptions and
laboratory practices, with the aim of offering a firm understanding
of how techniques and procedures work, as well as how to avoid
problems. Some major issues explored by the book's expert
contributors include:
* Working safely with biological samples and radioactive
materials
* DNA and RNA purification
* PCR
* Protein and nucleid acid hybridization
* Prokaryotic and eukaryotic expression systems
* Properly using and maintaining laboratory equipment
