The volumes in this series include contemporary techniques significant to a particular branch of neuroscience. They are an invaluable aid to the student as well as the experienced researcher not only in developing protocols in neuroscience but in disciplines where research is becoming closely related to neuroscience. Each volume of Methods in Neurosciences contains an index, and each chapter includes references. Dr. Conn became Editor-in-Chief of the series beginning with Volume 15, so each subsequent volume could be guest-edited by an expert in that specific field. This further strengthens the depth of coverage in Methods in Neurosciences for students and researchers alike.
- Comprehensive protocols included for: Enzymes involved in the activation of bioactive peptidases and proteins
- Prohormone/neuropeptide processing pathways
- Enzymes involved in peptide metabolism
- Posttranslational processing enzymes
Inhaltsverzeichnis
1;Cover;1 2;Table of Contents;6 3;Contributors to Volume 23;10 4;Preface;14 5;Volumes in Series;16 6;Section I: Molecular Approaches for the Study of Intracellular Processing Enzymes;18 6.1;Chapter 1. Molecular Strategies for Identifying Processing Enzymes;20 6.2;Chapter 2. In Situ Hybridization Techniques to Map Processing Enzymes;33 6.3;Chapter 3. Analysis of Ontogeny of Processing Enzyme Gene Expression and Regulation;62 6.4;Chapter 4. Use of Vaccinia Virus Vectors to Study Neuropeptide Processing;82 6.5;Chapter 5. Overexpression of Neuropeptide Precursors and Processing Enzymes;111 6.6;Chapter 6. Use of Antisense RNA to Block Peptide-Processing Enzyme Expression;126 7;Section II: Immunological and Biochemical Approaches to the Study of Peptide-Processing Pathways;140 7.1;Chapter 7. Combination of High-Performance Liquid Chromatography and Radioimmunoassay for Characterization of Peptide-Processing Pathways;142 7.2;Chapter 8. Development and Use of Two-Site Immunometric Assays for Examining Peptide-Processing Pathways;157 7.3;Chapter 9. Methods for Identification of Neuropeptide-Processing Pathways;172 7.4;Chapter 10. Immunological and Related Techniques for Studying Neurohypophyseal Peptide-Processing Pathways;212 7.5;Chapter 11. Approaches to Assessing Ontogeny of Processing Enzymes;225 7.6;Chapter 12. Measurement, Distribution, and Subcellular Localization of Peptide-Amidating Activity;236 7.7;Chapter 13. Methods for Studying Carboxypeptidase E;254 7.8;Chapter 14. Characterization of Endothelin-Converting Enzymes;268 7.9;Chapter 15. In Vivo Approaches for Studying Peptide Processing;283 8;Section III: Identification and Characterization of Extracellular Processing Enzymes in the Central Nervous System;296 8.1;Chapter 16. Identification and Characterization of Central Nervous Peptidase Activities;298 8.2;Chapter 17. Strategies for Characterizing, Cloning, and Expressing Soluble Endopeptidases;313 8.3;Chapter 18. Proteolytic Processing and Amyloid Protein Precur
sor of Alzheimer's Disease;334 8.4;Chapter 19. Strategies for Measurement of Angiotensin and Bradykinin Peptides and Their Metabolites in Central Nervous System and Other Tissues;345 8.5;Chapter 20. Distribution and Roles of Endopeptidase 24.11;361 8.6;Chapter 21. Identification and Distribution of Endopeptidase 24.16 in the Central Nervous System;380 8.7;Chapter 22. Autoradiographic Techniques to Map Angiotensin-Converting Enzyme in Brain and Other Tissues;400 9;Index;416 10;Color Plate Section;426
