This volume looks the current advanced protocols used to study aspects of the B cell receptor (BCR). The chapters in this book cover topics such as the mutant of BCR repertoire to understand antibody evolution; interactions between B cells and viruses; mechanical force during BCR activation; B cell signaling using flow cytometry; confocal microscopy, total internal reflection microscopy and intravital two-photon microscopy; and the methods used to study critical cell components related with B cell activation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and practical, B Cell Receptor Signaling: Methods and Protocols is a valuable resource for everyone in the scientific community currently working inthe B cell field.
Inhaltsverzeichnis
Activation-Induced Cytidine Deaminase Aided In Vitro Antibody Evolution. - Analyzing Mouse B-Cell Responses Specific to LCMV Infection. - Expression of Exogenous Genes in Murine Primary B Cells and B Cell Lines Using Retroviral Vectors. - Biophysical Techniques to Study B Cell Activation: Single Molecule Imaging and Force Measurements. - DNA-Based Probes for Measuring Mechanical Forces in Cell-Cell Contacts: Application to B Cell Antigen Extraction from Immune SynDeriving Quantitative Cell Biological Information from Dye-Dilution Lymphocyte Proliferation Experiments. - Flow Cytometry Analysis of mTOR Signaling in Antigen-Specific B Cells. - Ex Vivo Culture Assay to Measure Human Follicular Helper T (Tfh) Cell-Mediated Human B Cell Proliferation and Differentiation. - B Cell Receptor Signaling and Compartmentalization by Confocal Microscopy. - Investigate Early Activation Events of B Cell Receptor Signaling by Membrane Associated Antigen System and Total Internal Reflection Fluorescence MicroscopyImaging the Interactions between B Cells and Antigen-Presenting Cells. - In Vivo Tracking of Particulate Antigen Localization and Recognition by B Lymphocytes at Lymph Nodes. - Study B Cell Antigen Receptor Nano-scale Organization by In Situ Fab Proximity Ligation Assay. - Single Particle Tracking of Cell Surface Proteins. - The Use of Intravital Two-Photon and Thick Section Confocal Imaging to Analyze B Lymphocyte Trafficking in Lymph Nodes and Spleen. - Time-lapse Förster Resonance Energy Transfer Imaging by Confocal Laser Scanning Microscopy for Analyzing Dynamic Molecular Interactions in the Plasma Membrane of B Cells. - Understanding of B Cell Receptor Signaling through a Photo-Activatable Antigen Presentation System. - Use of Streptolysin O-induced Membrane Damage as a Method of Studying the Function of Lipid Rafts during B Cell Activation. - Visualization and Quantitative Analysis of the Actin Cytoskeleton upon B Cell Activation.
