Insect Cell Lines.- Development and characterization of insect cell lines.- New approaches to insect tissue culture.- Transgenic insect cells: mosquito cell mutants and the dihydrofolate reductase gene.- Insect cell physiology.- Insect cell cultivation: growth and kinetics.- Medium design for insect cell culture.- Viruses.- Baculovirus-insect cell interactions.- Replication patterns and cytopathology of cells infected with baculoviruses.- Construction of baculovirus recombinants.- Passage effect of virus infection in insect cells.- Post-translational modifications in insect cells.- Productivity of insect cells for recombinant proteins.- Chaperone and foldase coexpression in the baculovirus-insect cell expression system.- Engineering Aspects.- Shear sensitivity of insect cells.- Insect cell bioreactors.- Perfusion bioreactors for the production of recombinant proteins in insect cells.- Immobilization of insect cells.- Modelling baculovirus infection of insect cells in culture.- Scale up aspects of sparged insect-cell bioreactors.- Oxygen gradients in small and big sparged insect-cell bioreactors.- Downstream processing of insect cell cultures.- Applications.- Parvovirus diagnostics and vaccine production in insect cells.- Classical swine fever virus diagnostics and vaccine production in insect cells.- Production of multidomain complement glycoproteins in insect cells.- Economic and Regulatory Aspects.- Economics of baculovirus-insect cell production systems.- Safety aspects of insect cell culture.- Regulatory issues in the use of insect-cell culture.
Inhaltsverzeichnis
Insect Cell Lines. - Development and characterization of insect cell lines. - New approaches to insect tissue culture. - Transgenic insect cells: mosquito cell mutants and the dihydrofolate reductase gene. - Insect cell physiology. - Insect cell cultivation: growth and kinetics. - Medium design for insect cell culture. - Viruses. - Baculovirus-insect cell interactions. - Replication patterns and cytopathology of cells infected with baculoviruses. - Construction of baculovirus recombinants. - Passage effect of virus infection in insect cells. - Post-translational modifications in insect cells. - Productivity of insect cells for recombinant proteins. - Chaperone and foldase coexpression in the baculovirus-insect cell expression system. - Engineering Aspects. - Shear sensitivity of insect cells. - Insect cell bioreactors. - Perfusion bioreactors for the production of recombinant proteins in insect cells. - Immobilization of insect cells. - Modelling baculovirus infection of insect cells in culture. - Scale up aspects of sparged insect-cell bioreactors. - Oxygen gradients in small and big sparged insect-cell bioreactors. - Downstream processing of insect cell cultures. - Applications. - Parvovirus diagnostics and vaccine production in insect cells. - Classical swine fever virus diagnostics and vaccine production in insect cells. - Production of multidomain complement glycoproteins in insect cells. - Economic and Regulatory Aspects. - Economics of baculovirus-insect cell production systems. - Safety aspects of insect cell culture. - Regulatory issues in the use of insect-cell culture.
