Ionotropic Glutamate Receptor Technologies

Concise and easy-to-use, Ionotropic Glutamate Receptor Technologies aims to facilitate the implementation of specific methods to iGluR investigations.

Assaying AMPA Receptor Oligomerization. - A Step-by-Step Guide to Single Subunit Counting Of Membrane-Bound Proteins in Mammalian Cells. - Counting NMDA Receptors at the Cell Surface. - Electron Microscopy Analysis of AMPA receptors in Dendritic Spines. - Electrophysiological Tagging of Ionotropic Receptors. - Functional Detection of Novel Triheteromeric NMDA Receptors. - Calcium Imaging to Study NMDA Receptor-mediated Cellular Responses. - NMR Approaches to Functional Dynamics Of Genetically Separated iGluR Domains. - Computing Conformational Free Energies of iGluR Ligand-Binding Domains. - LRET Methods for Investigating Conformational Changes in Functional Ionotropic Glutamate Receptors. - Assaying the Energetics of NMDA Receptor Pore Opening. - Constructing a Rapid Solution Exchange System. - Assessing Effects of Ligand-Binding Mutations To AMPA And Kainate Receptor Kinetics. - Analysis of Whole-Cell NMDA Receptor Responses. - Expression, Purification and Crystallization of Full Length Ionotropic Glutamate Receptors. - Timing AMPA Receptor Activation with Laser-pulse Photolysis. - Current Recording and Kinetic Analyses for Single AMPA Receptors. - Extracting Rate Constants for NMDA Receptor Gating from One-channel Current Recordings.